PMID- 10369260
OWN - NLM
STAT- MEDLINE
DCOM- 19990629
LR  - 20071114
IS  - 1061-4036 (Print)
IS  - 1061-4036 (Linking)
VI  - 22
IP  - 2
DP  - 1999 Jun
TI  - A double-stranded RNA binding protein required for activation of repressed
      messages in mammalian germ cells.
PG  - 171-4
AB  - Chromatin packaging in mammalian spermatozoa requires an ordered replacement of
      the somatic histones by two classes of spermatid-specific basic proteins, the
      transition proteins and the protamines. Temporal expression of transition
      proteins and protamines during spermatid differentiation is under translational
      control, and premature translation of protamine 1 leads to precocious nuclear
      condensation and sterility. We have previously suggested that the double-stranded
      (ds) RNA binding protein Prbp (encoded by the gene Tarbp2) functions as a
      translational regulator during mouse spermatogenesis. Here we show that Prbp is
      required for proper translational activation of the mRNAs encoding the
      protamines. We generated mice that carry a targeted disruption of Tarbp2 and
      determined that they were sterile and severely oligospermic. Using
      immunohistological analysis, we determined that the endogenous Prm2 mRNA and a
      reporter mRNA carrying protamine 1 translational-control elements were translated
      in a mosaic pattern. We showed that failure to synthesize the protamines resulted
      in delayed replacement of the transition proteins and subsequent failure of
      spermiation. The timing of Prbp expression suggests that it may function as a
      chaperone in the assembly of specific translationally regulated ribonucleoprotein
      particles.
FAU - Zhong, J
AU  - Zhong J
AD  - Department of Genetics, University of Washington, Seattle 98195, USA.
FAU - Peters, A H
AU  - Peters AH
FAU - Lee, K
AU  - Lee K
FAU - Braun, R E
AU  - Braun RE
LA  - eng
GR  - HD27215/HD/NICHD NIH HHS/United States
GR  - U54 HD12629/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Genet
JT  - Nature genetics
JID - 9216904
RN  - 0 (RNA-Binding Proteins)
RN  - 136628-24-5 (trans-activation responsive RNA-binding protein)
SB  - IM
MH  - Animals
MH  - Body Weight
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred Strains
MH  - Mice, Knockout
MH  - Organ Size
MH  - RNA-Binding Proteins/*genetics/*metabolism
MH  - Sperm Count
MH  - Testis/anatomy & histology/cytology/*metabolism
EDAT- 1999/06/16 10:00
MHDA- 2001/03/23 10:01
CRDT- 1999/06/16 10:00
PHST- 1999/06/16 10:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/06/16 10:00 [entrez]
AID - 10.1038/9684 [doi]
PST - ppublish
SO  - Nat Genet. 1999 Jun;22(2):171-4. doi: 10.1038/9684.