PMID- 10368977
OWN - NLM
STAT- MEDLINE
DCOM- 19990716
LR  - 20151119
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 13
IP  - 11
DP  - 1999
TI  - Characterization of tyrosine sulfate residues in antihemophilic recombinant
      factor VIII by liquid chromatography electrospray ionization tandem mass
      spectrometry and amino acid analysis.
PG  - 1016-23
AB  - Recombinant Factor VIII (rFVIII) is involved in the cascade of biochemical
      reactions leading to blood coagulation and is used for the treatment of
      haemophilia A. Plasma-derived FVIII (pdFVIII) has been reported to be
      post-translationally modified by sulfation of tyrosine residues at positions 346,
      1664, 1680, 718, 719 and 723. This report describes the quantitation of tyrosine 
      sulfate residues in BHK-derived, human rFVIII by amino acid composition analysis 
      and the identification of their positions in the polypeptide sequence using a
      combination of liquid chromatography and electrospray ionization mass
      spectrometry in the analysis of proteolytic digests of the protein.
FAU - Severs, J C
AU  - Severs JC
AD  - Bayer Pharmaceuticals, Berkeley, CA 94701, USA.
FAU - Carnine, M
AU  - Carnine M
FAU - Eguizabal, H
AU  - Eguizabal H
FAU - Mock, K K
AU  - Mock KK
LA  - eng
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Amino Acids)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - 29166358BF (tyrosine O-sulfate)
RN  - 42HK56048U (Tyrosine)
RN  - 9001-27-8 (Factor VIII)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acids/analysis
MH  - Chromatography, High Pressure Liquid
MH  - Factor VIII/*analysis
MH  - Hydrolysis
MH  - Mass Spectrometry
MH  - Molecular Sequence Data
MH  - Peptide Fragments/analysis
MH  - Peptide Mapping
MH  - Recombinant Proteins/analysis
MH  - Spectrophotometry, Ultraviolet
MH  - Thrombin/chemistry
MH  - Tyrosine/*analogs & derivatives/analysis
EDAT- 1999/06/16 10:00
MHDA- 2000/06/20 09:00
CRDT- 1999/06/16 10:00
PHST- 1999/06/16 10:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/06/16 10:00 [entrez]
AID - 10.1002/(SICI)1097-0231(19990615)13:11<1016::AID-RCM599>3.0.CO;2-5 [pii]
AID - 10.1002/(SICI)1097-0231(19990615)13:11<1016::AID-RCM599>3.0.CO;2-5 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 1999;13(11):1016-23. doi:
      10.1002/(SICI)1097-0231(19990615)13:11<1016::AID-RCM599>3.0.CO;2-5.