PMID- 10367892
OWN - NLM
STAT- MEDLINE
DCOM- 19990629
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 97
IP  - 5
DP  - 1999 May 28
TI  - Structural view of the Ran-Importin beta interaction at 2.3 A resolution.
PG  - 635-46
AB  - Transport receptors of the Importin beta family shuttle between the nucleus and
      cytoplasm and mediate transport of macromolecules through nuclear pore complexes.
      They interact specifically with the GTP-binding protein Ran, which in turn
      regulates their interaction with cargo. Here, we report the three-dimensional
      structure of a complex between Ran bound to the nonhydrolyzable GTP analog GppNHp
      and a 462-residue fragment from Importin beta. The structure of Importin beta
      shows 10 tandem repeats resembling HEAT and Armadillo motifs. They form an
      irregular crescent, the concave site of which forms the interface with
      Ran-triphosphate. The importin-binding site of Ran does not overlap with that of 
      the Ran-binding domain of RanBP2.
FAU - Vetter, I R
AU  - Vetter IR
AD  - Max-Planck-Institut fur molekulare Physiologie, Dortmund, Germany.
FAU - Arndt, A
AU  - Arndt A
FAU - Kutay, U
AU  - Kutay U
FAU - Gorlich, D
AU  - Gorlich D
FAU - Wittinghofer, A
AU  - Wittinghofer A
LA  - eng
SI  - PDB/1IBR
PT  - Journal Article
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Karyopherins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Recombinant Proteins)
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Consensus Sequence
MH  - Crystallography, X-Ray
MH  - Drosophila
MH  - GTP-Binding Proteins/chemistry/metabolism
MH  - Guanylyl Imidodiphosphate/metabolism
MH  - Humans
MH  - Karyopherins
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*chemistry/metabolism
MH  - Oryza
MH  - Protein Isoforms/chemistry/metabolism
MH  - Protein Structure, Secondary
MH  - Protein Structure, Tertiary
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Saccharomyces cerevisiae
MH  - Schizosaccharomyces
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - ran GTP-Binding Protein
EDAT- 1999/06/15 00:00
MHDA- 1999/06/15 00:01
CRDT- 1999/06/15 00:00
PHST- 1999/06/15 00:00 [pubmed]
PHST- 1999/06/15 00:01 [medline]
PHST- 1999/06/15 00:00 [entrez]
AID - S0092-8674(00)80774-6 [pii]
AID - 10.1016/s0092-8674(00)80774-6 [doi]
PST - ppublish
SO  - Cell. 1999 May 28;97(5):635-46. doi: 10.1016/s0092-8674(00)80774-6.