PMID- 10366780
OWN - NLM
STAT- MEDLINE
DCOM- 19990719
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1438
IP  - 3
DP  - 1999 Jun 10
TI  - A porcine homolog of the major secretory protein of human epididymis, HE1,
      specifically binds cholesterol.
PG  - 377-87
AB  - A porcine homolog of the major secretory protein of human epididymis, HE1, was
      for the first time purified from the porcine cauda epididymal fluid. The HE1
      homolog was secreted into the epididymal fluid as a 19-kDa glycoprotein, whose
      sugar moiety was gradually processed to form a 16-kDa protein during transit
      through the epididymis. The HE1 homolog mRNA was detected only in the caput and
      corpus epididymis among the porcine tissues examined. The purified HE1 homolog
      specifically bound cholesterol with high affinity (Kd=2. 3 microM). The binding
      stoichiometry was determined to be 0.94 mol/mol, suggesting that 1 mol of
      cholesterol binds to 1 mol of the protein. It was also found that the HE1 homolog
      is a major cholesterol-binding protein in the porcine epididymal fluid. The
      possibility that the HE1 homolog is involved in the regulation of the lipid
      composition of the sperm membranes during the maturation in epididymis is
      discussed.
FAU - Okamura, N
AU  - Okamura N
AD  - Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305,
      Japan. naooka@igaku.md.tsukuba.ac.jp
FAU - Kiuchi, S
AU  - Kiuchi S
FAU - Tamba, M
AU  - Tamba M
FAU - Kashima, T
AU  - Kashima T
FAU - Hiramoto, S
AU  - Hiramoto S
FAU - Baba, T
AU  - Baba T
FAU - Dacheux, F
AU  - Dacheux F
FAU - Dacheux, J L
AU  - Dacheux JL
FAU - Sugita, Y
AU  - Sugita Y
FAU - Jin, Y Z
AU  - Jin YZ
LA  - eng
SI  - GENBANK/U62253
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Glycoproteins)
RN  - 0 (NPC2 protein, human)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.4.21.36 (Pancreatic Elastase)
RN  - EC 3.4.21.36 (cholesterol-binding protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Body Fluids/metabolism
MH  - Carrier Proteins/isolation & purification
MH  - Cholesterol/*metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/metabolism
MH  - Epididymis/chemistry/*metabolism
MH  - Gene Library
MH  - Glycoproteins/isolation & purification/*metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Pancreatic Elastase
MH  - Proteins
MH  - RNA, Messenger/analysis
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Sperm Maturation
MH  - Spermatozoa/metabolism
MH  - Swine
EDAT- 1999/06/15 00:00
MHDA- 1999/06/15 00:01
CRDT- 1999/06/15 00:00
PHST- 1999/06/15 00:00 [pubmed]
PHST- 1999/06/15 00:01 [medline]
PHST- 1999/06/15 00:00 [entrez]
AID - S1388-1981(99)00070-0 [pii]
AID - 10.1016/s1388-1981(99)00070-0 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Jun 10;1438(3):377-87. doi:
      10.1016/s1388-1981(99)00070-0.