PMID- 10366737
OWN - NLM
STAT- MEDLINE
DCOM- 19990803
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 69
IP  - 2
DP  - 1999 Jun 8
TI  - Effects of kainate-mediated excitotoxicity on the expression of rat counterparts 
      of A170 and MSP23 stress proteins in the brain.
PG  - 155-63
AB  - Stress proteins play important roles in the protective mechanisms under critical 
      conditions for cell survival. We report here the expression of A170 and MSP23,
      oxidative stress-inducible proteins, under kainate-mediated excitotoxicity in the
      rat brain. A170 mRNA was significantly induced in the brain 5-8 h after i.p.
      kainate administration. MSP23 mRNA was observed at quite a low level in the rat
      brain, and the induction of MSP23 mRNA was not observed during the period 24 h
      after kainate administration. Immunoblot analysis demonstrated that the maximal
      expression level of A170 protein occurred 8 h after treatment in each part of the
      brain. MSP23 protein was constitutively expressed in the brain and the level of
      this protein was significantly decreased during the period 24 h after kainate
      administration. In situ hybridization and immunohistochemical studies showed that
      A170 was expressed predominantly in neurons, especially in pyramidal neurons of
      the cerebrum and cerebellar Purkinje cells, while MSP23 was expressed in
      oligodendrocytes. The induction of A170 was observed in the regions which are
      affected by excitotoxicity and this induction was observed in the earlier phase
      than cell death. Also, the region which shows high vulnerability to
      excitotoxicity such as pyramidal cell layer in the hippocampus, showed lower A170
      expression than that which shows resistance to excitotoxicity, such as the
      dentate gyrus in the hippocampus. These results suggest that A170 may play a
      protective role in the brain under kainate-mediated excitotoxicity.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Nakaso, K
AU  - Nakaso K
AD  - Department of Biochemistry, Faculty of Medicine, Tottori University, Yonago,
      Japan. kazuhiro@grapemed.tottori-u.ac.jp
FAU - Kitayama, M
AU  - Kitayama M
FAU - Ishii, T
AU  - Ishii T
FAU - Bannai, S
AU  - Bannai S
FAU - Yanagawa, T
AU  - Yanagawa T
FAU - Kimura, K
AU  - Kimura K
FAU - Nakashima, K
AU  - Nakashima K
FAU - Ohama, E
AU  - Ohama E
FAU - Yamada, K
AU  - Yamada K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Sequestosome-1 Protein)
RN  - 0 (Sqstm1 protein, rat)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.14 (Prdx1 protein, rat)
RN  - EC 1.11.1.15 (Peroxiredoxins)
RN  - EC 1.11.1.15 (Prdx1 protein, mouse)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/metabolism
MH  - Heat-Shock Proteins/biosynthesis
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Kainic Acid/*pharmacology
MH  - Male
MH  - Nerve Tissue Proteins/*biosynthesis
MH  - Oxidative Stress/*physiology
MH  - *Peroxidases
MH  - Peroxiredoxins
MH  - Rats
MH  - Rats, Wistar
MH  - Sequestosome-1 Protein
EDAT- 1999/06/15 00:00
MHDA- 1999/06/15 00:01
CRDT- 1999/06/15 00:00
PHST- 1999/06/15 00:00 [pubmed]
PHST- 1999/06/15 00:01 [medline]
PHST- 1999/06/15 00:00 [entrez]
AID - S0169328X99000935 [pii]
AID - 10.1016/s0169-328x(99)00093-5 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 Jun 8;69(2):155-63. doi:
      10.1016/s0169-328x(99)00093-5.