PMID- 10366582
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 258
IP  - 2
DP  - 1999 Jun 5
TI  - Isolation of a human endogenous retroviral HERV-H element with an open env
      reading frame.
PG  - 441-50
AB  - About 100 elements of the human endogenous retroviral HERV-H family have
      full-length env genes potentially coding for Env proteins with sequences highly
      similar to the immunosuppressive peptide CKS-17 from the MLV transmembrane
      protein p15E. However, previously sequenced HERV-H env genes have contained stop 
      codons or framehifts. To isolate elements with open env reading frames, we first 
      tried to assess the diversity of HERV-H env genes by comparing PCR-generated env 
      sequences from genomic DNA with published HERV-H sequences. A region at the
      beginning of env displayed a similarity of 84-98% among 15 different elements. We
      then used a probe from one of the PCR-generated clones, 98% similar to the
      consensus sequence in this region, to screen a human genomic lambda library.
      Three HERV-H elements displaying ca. 98% identity in the env gene were isolated
      and were shown to have integrated relatively recently, after the divergence of
      the orangutan and the african great ape lineages. One of these elements,
      HERV-H19, had a 1752-bp open env reading frame, producing a 77-kDa Env protein in
      in vitro translation reactions. This is the first demonstration of a coding
      competent member of the HERV-H family. These findings raise the possibility that 
      HERV-H Env proteins may play a biological role in human cells.
CI  - Copyright 1999 Academic Press.
FAU - Lindeskog, M
AU  - Lindeskog M
AD  - Section of Virology, Lund University, Solvegatan 23, Lund, S-22362, Sweden.
      mats.lindeskog@mmb.lu.se
FAU - Mager, D L
AU  - Mager DL
FAU - Blomberg, J
AU  - Blomberg J
LA  - eng
SI  - GENBANK/AF108838
SI  - GENBANK/AF108839
SI  - GENBANK/AF108840
SI  - GENBANK/AF108841
SI  - GENBANK/AF108842
SI  - GENBANK/AF108843
SI  - GENBANK/AH007765
SI  - GENBANK/AH007766
SI  - GENBANK/AH007767
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Viral
MH  - Endogenous Retroviruses/*genetics
MH  - *Genes, env
MH  - Hominidae/virology
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Open Reading Frames
MH  - Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
EDAT- 1999/06/15 00:00
MHDA- 1999/06/15 00:01
CRDT- 1999/06/15 00:00
PHST- 1999/06/15 00:00 [pubmed]
PHST- 1999/06/15 00:01 [medline]
PHST- 1999/06/15 00:00 [entrez]
AID - S0042-6822(99)99750-7 [pii]
AID - 10.1006/viro.1999.9750 [doi]
PST - ppublish
SO  - Virology. 1999 Jun 5;258(2):441-50. doi: 10.1006/viro.1999.9750.