PMID- 10366447
OWN - NLM
STAT- MEDLINE
DCOM- 19990816
LR  - 20061115
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 58
IP  - 2
DP  - 1999 Jun 1
TI  - cDNA cloning, genomic structure, and chromosomal localization of a novel murine
      epidermis-type lipoxygenase.
PG  - 158-64
AB  - Using a combination of degenerate PCR technique and conventional screening
      procedures, we isolated a cDNA encoding a novel lipoxygenase, termed
      epidermis-type lipoxygenase-3 (e-LOX-3, gene symbol Aloxe3), from mouse skin.
      Aloxe3 mRNA is expressed in the stratified epithelia of skin, tongue, and
      forestomach. The cDNA encodes a protein of 711 amino acids with a calculated
      molecular mass of 80.6 kDa. The amino acid sequence shows approximately 54%
      identity to the recently identified 12(R)-lipoxygenase. Sequence comparison
      revealed a segment of 41 amino acid residues localized near the boundary between 
      the N- and the C-terminal domain sequences of the molecule, a structural feature 
      that is also characteristic of 12(R)-lipoxygenase, suggesting that these two
      epidermis-derived lipoxygenases may be members of a novel structural class of
      mammalian lipoxygenases. The novel lipoxygenase gene is divided into 15 exons and
      14 introns, spanning 22.3 kb of genomic DNA. By interspecific backcross analysis,
      the novel gene was localized to the central region of mouse chromosome 11.
CI  - Copyright 1999 Academic Press.
FAU - Kinzig, A
AU  - Kinzig A
AD  - Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Im
      Neuenheimer Feld 280, Heidelberg, 69120, Germany.
FAU - Heidt, M
AU  - Heidt M
FAU - Furstenberger, G
AU  - Furstenberger G
FAU - Marks, F
AU  - Marks F
FAU - Krieg, P
AU  - Krieg P
LA  - eng
SI  - GENBANK/J51789
SI  - GENBANK/Y14695
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - EC 1.13.11.12 (Lipoxygenase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Epidermis/*metabolism
MH  - Exons
MH  - Gene Library
MH  - Introns
MH  - Lipoxygenase/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Tissue Distribution
EDAT- 1999/06/15 00:00
MHDA- 1999/06/15 00:01
CRDT- 1999/06/15 00:00
PHST- 1999/06/15 00:00 [pubmed]
PHST- 1999/06/15 00:01 [medline]
PHST- 1999/06/15 00:00 [entrez]
AID - S0888-7543(99)95816-0 [pii]
AID - 10.1006/geno.1999.5816 [doi]
PST - ppublish
SO  - Genomics. 1999 Jun 1;58(2):158-64. doi: 10.1006/geno.1999.5816.