PMID- 10366446
OWN - NLM
STAT- MEDLINE
DCOM- 19990816
LR  - 20190524
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 58
IP  - 2
DP  - 1999 Jun 1
TI  - Novel proteins interacting with the leucine-rich repeat domain of human
      flightless-I identified by the yeast two-hybrid system.
PG  - 146-57
AB  - The flightless-I gene encodes a member of the gelsolin-like family of
      actin-binding proteins linked to a leucine-rich repeat (LRR) domain. It is
      required for cellularization during early embryogenesis and normal development of
      the indirect flight muscles in Drosophila melanogaster. Although the association 
      between actin and the gelsolin-like domain of the human Flightless-I homologue
      (FLI) has been established, its biological role is unknown. The human FLI gene is
      mapped within the Smith-Magenis microdeletion region of chromosome 17. We report 
      the identification of two related genes, LRRFIP1 and LRRFIP2, encoding proteins
      that interact with the LRR domain of human FLI using the yeast two-hybrid system.
      LRRFIP1 exhibits sequence identity with the TRIP RNA-binding protein and GCF-2
      transcriptional repressor, which are also related to the murine FLAP-1 gene.
      LRRFIP2 is a novel gene that shares sequence homology with LRRFIP1 and FLAP-1.
      LRRFIP1 and LRRFIP2 both express alternative splice variants in heart and
      skeletal muscle tissue. A coiled-coil domain, conserved within each encoded
      protein, serves as a potential interaction motif for FLI LRR. The occurrence of
      multiple proteins able to interact with FLI within the same tissue suggests that 
      they may compete for the same binding site. Sequencing and PCR-directed genomic
      analysis indicate that LRRFIP1 and LRRFIP2 are related genes that arose from gene
      duplication.
CI  - Copyright 1999 Academic Press.
FAU - Fong, K S
AU  - Fong KS
AD  - Department of Zoology, University of Hawaii at Manoa, Honolulu, Hawaii, 96822,
      USA.
FAU - de Couet, H G
AU  - de Couet HG
LA  - eng
SI  - GENBANK/AF115509
SI  - GENBANK/AF115510
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Adenovirus E3 Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Gelsolin)
RN  - 0 (Insect Proteins)
RN  - 0 (LRRFIP1 protein, human)
RN  - 0 (LRRFIP2 protein, human)
RN  - 0 (OPTN protein, human)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factor TFIIIA)
RN  - 0 (fliI protein, Drosophila)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - *Adenovirus E3 Proteins
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Carrier Proteins/*genetics/metabolism
MH  - Cloning, Molecular
MH  - Computer Simulation
MH  - *Drosophila Proteins
MH  - *Gelsolin
MH  - Gene Library
MH  - Humans
MH  - Insect Proteins/*genetics
MH  - Leucine/*metabolism
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - RNA-Binding Proteins/*genetics/metabolism
MH  - Recombinant Fusion Proteins
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - *Transcription Factor TFIIIA
EDAT- 1999/06/15 00:00
MHDA- 1999/06/15 00:01
CRDT- 1999/06/15 00:00
PHST- 1999/06/15 00:00 [pubmed]
PHST- 1999/06/15 00:01 [medline]
PHST- 1999/06/15 00:00 [entrez]
AID - S0888-7543(99)95817-2 [pii]
AID - 10.1006/geno.1999.5817 [doi]
PST - ppublish
SO  - Genomics. 1999 Jun 1;58(2):146-57. doi: 10.1006/geno.1999.5817.