PMID- 10364558
OWN - NLM
STAT- MEDLINE
DCOM- 19990625
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 284
IP  - 5421
DP  - 1999 Jun 11
TI  - Crystal structure of the Zalpha domain of the human editing enzyme ADAR1 bound to
      left-handed Z-DNA.
PG  - 1841-5
AB  - The editing enzyme double-stranded RNA adenosine deaminase includes a DNA binding
      domain, Zalpha, which is specific for left-handed Z-DNA. The 2.1 angstrom crystal
      structure of Zalpha complexed to DNA reveals that the substrate is in the
      left-handed Z conformation. The contacts between Zalpha and Z-DNA are made
      primarily with the "zigzag" sugar-phosphate backbone, which provides a basis for 
      the specificity for the Z conformation. A single base contact is observed to
      guanine in the syn conformation, characteristic of Z-DNA. Intriguingly, the
      helix-turn-helix motif, frequently used to recognize B-DNA, is used by Zalpha to 
      contact Z-DNA.
FAU - Schwartz, T
AU  - Schwartz T
AD  - Department of Biology, Massachusetts Institute of Technology, Cambridge, MA
      02139, USA.
FAU - Rould, M A
AU  - Rould MA
FAU - Lowenhaupt, K
AU  - Lowenhaupt K
FAU - Herbert, A
AU  - Herbert A
FAU - Rich, A
AU  - Rich A
LA  - eng
SI  - PDB/1QBJ
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (RNA-Binding Proteins)
RN  - 059QF0KO0R (Water)
RN  - 9007-49-2 (DNA)
RN  - EC 3.5.4.4 (ADARB1 protein, human)
RN  - EC 3.5.4.4 (Adenosine Deaminase)
SB  - IM
SB  - S
MH  - Adenosine Deaminase/*chemistry/metabolism
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Crystallography, X-Ray
MH  - DNA/chemistry/*metabolism
MH  - Helix-Turn-Helix Motifs
MH  - Humans
MH  - Hydrogen Bonding
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Protein Conformation
MH  - Protein Structure, Secondary
MH  - RNA-Binding Proteins
MH  - Substrate Specificity
MH  - Water/metabolism
OTO - NASA
OT  - Non-programmatic
EDAT- 1999/06/12 00:00
MHDA- 1999/06/12 00:01
CRDT- 1999/06/12 00:00
PHST- 1999/06/12 00:00 [pubmed]
PHST- 1999/06/12 00:01 [medline]
PHST- 1999/06/12 00:00 [entrez]
AID - 10.1126/science.284.5421.1841 [doi]
PST - ppublish
SO  - Science. 1999 Jun 11;284(5421):1841-5. doi: 10.1126/science.284.5421.1841.