PMID- 10364477
OWN - NLM
STAT- MEDLINE
DCOM- 19990716
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 259
IP  - 3
DP  - 1999 Jun 16
TI  - The endothelial cell protein C receptor (EPCR) functions as a primary receptor
      for protein C activation on endothelial cells in arteries, veins, and
      capillaries.
PG  - 671-7
AB  - Plasma protein C functions as an anticoagulant when it is converted to the active
      form of serine protease. Protein C activation has been found to be mediated by
      the endothelial cell surface thrombin/thrombomodulin (TM) complex. In addition,
      we recently identified the endothelial cell protein C/activated protein C
      receptor (EPCR) which is capable of high-affinity binding for protein C. In this 
      study, we established monoclonal antibodies (mAbs) against EPCR including several
      function blocking antibodies. Immunohistochemical analysis using these mAbs
      demonstrated that EPCR is widely expressed in the endothelial cells of arteries, 
      veins, and capillaries in the lung, heart, and skin. Function blocking anti-EPCR 
      mAbs strongly inhibited protein C activation mediated by primary cultured
      arterial endothelial cells which express abundant EPCR. Anti-EPCR mAbs also
      prevent protein C activation mediated by microvascular endothelial cells. These
      results indicate that EPCR functions as an important regulator for the protein C 
      pathway in various types of vessels.
CI  - Copyright 1999 Academic Press.
FAU - Ye, X
AU  - Ye X
AD  - Department of Immunology, Saga Medical School, Nabeshima, 849-8501, Japan.
FAU - Fukudome, K
AU  - Fukudome K
FAU - Tsuneyoshi, N
AU  - Tsuneyoshi N
FAU - Satoh, T
AU  - Satoh T
FAU - Tokunaga, O
AU  - Tokunaga O
FAU - Sugawara, K
AU  - Sugawara K
FAU - Mizokami, H
AU  - Mizokami H
FAU - Kimoto, M
AU  - Kimoto M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Blood Coagulation Factors)
RN  - 0 (Protein C)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (activated protein C receptor)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Arteries/*metabolism
MH  - *Blood Coagulation Factors
MH  - Capillaries/*metabolism
MH  - Cell Line
MH  - Dose-Response Relationship, Drug
MH  - Endothelium, Vascular/*metabolism
MH  - Heart/anatomy & histology
MH  - Humans
MH  - Kinetics
MH  - Liver/anatomy & histology
MH  - Lung/anatomy & histology
MH  - Protein C/*metabolism
MH  - Receptors, Cell Surface/analysis/immunology/*physiology
MH  - Veins/*metabolism
MH  - Vena Cava, Inferior/anatomy & histology
EDAT- 1999/06/12 00:00
MHDA- 1999/06/12 00:01
CRDT- 1999/06/12 00:00
PHST- 1999/06/12 00:00 [pubmed]
PHST- 1999/06/12 00:01 [medline]
PHST- 1999/06/12 00:00 [entrez]
AID - 10.1006/bbrc.1999.0846 [doi]
AID - S0006-291X(99)90846-X [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jun 16;259(3):671-7. doi:
      10.1006/bbrc.1999.0846.