PMID- 10364453
OWN - NLM
STAT- MEDLINE
DCOM- 19990716
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 259
IP  - 3
DP  - 1999 Jun 16
TI  - Identification and characterization of a myristylated and palmitylated
      serine/threonine protein kinase.
PG  - 533-8
AB  - We report the molecular cloning and initial characterization of a novel fatty
      acid acylated serine/threonine protein kinase. The putative open reading frame is
      predicted to encode a 305 amino acid protein possessing a carboxy-terminal
      protein kinase domain and amino-terminal myristylation and palmitylation sites.
      The protein kinase has been accordingly denoted as the myristylated and
      palmitylated serine/threonine protein kinase (MPSK). Human and mouse MPSKs share 
      approximately 93% identity at the amino acid level with complete retention of
      acylation sites. Radiation hybridization localized the human MPSK gene to
      chromosome 2q34-37. Northern analysis demonstrated that the human MPSK 1.7
      kilobase mRNA is widely distributed. Epitope tagged human MPSK was found to be
      acylated by myristic acid at glycine residue 2 and by palmitic acid at cysteines 
      6 and/or 8. Palmitylation of MPSK in these experiments was found to require an
      intact myristylation site. While epitope tagged MPSK in immune complexes or
      purified human glutathione S transferase-MPSK was found to autophosphorylate at
      one or more threonine residues, the enzyme was not found to phosphorylate several
      other common exogenous substrates. Indeed, only PHAS-I was identified as an
      exogenous substrate which was found to be phosphorylated on threonine and serine 
      residues.
CI  - Copyright 1999 Academic Press.
FAU - Berson, A E
AU  - Berson AE
AD  - Department of Cellular Signaling, DNAX Research Institute, Palo Alto, California,
      94304, USA.
FAU - Young, C
AU  - Young C
FAU - Morrison, S L
AU  - Morrison SL
FAU - Fujii, G H
AU  - Fujii GH
FAU - Sheung, J
AU  - Sheung J
FAU - Wu, B
AU  - Wu B
FAU - Bolen, J B
AU  - Bolen JB
FAU - Burkhardt, A L
AU  - Burkhardt AL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Palmitates)
RN  - 0 (Transcription Factors)
RN  - 0I3V7S25AW (Myristic Acid)
RN  - EC 2.7.1.- (Stk16 protein, mouse)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (STK16 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 2
MH  - Humans
MH  - Immunoblotting
MH  - Mice
MH  - Molecular Sequence Data
MH  - Myristic Acid/metabolism
MH  - Palmitates/metabolism
MH  - Physical Chromosome Mapping
MH  - Protein-Serine-Threonine Kinases/*chemistry
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - *Transcription Factors
EDAT- 1999/06/12 00:00
MHDA- 1999/06/12 00:01
CRDT- 1999/06/12 00:00
PHST- 1999/06/12 00:00 [pubmed]
PHST- 1999/06/12 00:01 [medline]
PHST- 1999/06/12 00:00 [entrez]
AID - 10.1006/bbrc.1999.0811 [doi]
AID - S0006-291X(99)90811-2 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jun 16;259(3):533-8. doi:
      10.1006/bbrc.1999.0811.