PMID- 10364449
OWN - NLM
STAT- MEDLINE
DCOM- 19990716
LR  - 20161124
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 259
IP  - 3
DP  - 1999 Jun 16
TI  - DNA binding and transactivating properties of the paired and homeobox protein
      Pax4.
PG  - 510-8
AB  - Transcription factors Pax-4 and Pax-6 are known to be key regulators of
      pancreatic cell differentiation and development. We report on the cloning of a
      mouse Pax-4 gene, which contains 10 exons, spanning a 4. 7-kbp region. The
      gene-targeting experiments revealed that Pax-4 and Pax-6 cannot substitute for
      each other in tissue with overlapping expression of both genes. We identified
      DNA-binding specificities of Pax-4 paired domain and paired-type homeodomain.
      Despite the different Pax-4 amino acid residues in positions responsible for
      Pax-6 paired-domain specificity, the DNA-binding specificities of Pax-4 and Pax-6
      are similar. The Pax-4 homeodomain was shown to preferentially dimerize on DNA
      sequences consisting of an inverted TAAT motif, separated by 4-nucleotide
      spacing. The Pax-4 transactivation domain was localized within its C-terminal
      region, which transactivated GAL-based reporter 2.5-fold less than the C-terminal
      region of Pax-6. We believe that Pax-4 can act as a Pax-6 "repressor," due to the
      competition for binding sites and lower transactivation potential of Pax-4.
CI  - Copyright 1999 Academic Press.
FAU - Kalousova, A
AU  - Kalousova A
AD  - Institute of Molecular Genetics, Academy of Sciences of the Czech Republic,
      Prague, Czech Republic. Anna@biomed.cas.cz
FAU - Benes, V
AU  - Benes V
FAU - Paces, J
AU  - Paces J
FAU - Paces, V
AU  - Paces V
FAU - Kozmik, Z
AU  - Kozmik Z
LA  - eng
SI  - GENBANK/AF142321
SI  - GENBANK/AF145233
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (PAX4 protein, human)
RN  - 0 (PAX6 Transcription Factor)
RN  - 0 (PAX6 protein, human)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (Pax4 protein, mouse)
RN  - 0 (Pax6 protein, mouse)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Exons
MH  - Eye Proteins
MH  - Homeodomain Proteins/*physiology
MH  - Humans
MH  - Introns
MH  - Kidney/metabolism
MH  - Luciferases/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - PAX6 Transcription Factor
MH  - Paired Box Transcription Factors
MH  - Repressor Proteins
MH  - Sequence Homology, Amino Acid
MH  - Transcription Factors/*physiology
MH  - Transcriptional Activation/*physiology
EDAT- 1999/06/12 00:00
MHDA- 1999/06/12 00:01
CRDT- 1999/06/12 00:00
PHST- 1999/06/12 00:00 [pubmed]
PHST- 1999/06/12 00:01 [medline]
PHST- 1999/06/12 00:00 [entrez]
AID - 10.1006/bbrc.1999.0809 [doi]
AID - S0006291X99908094 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jun 16;259(3):510-8. doi:
      10.1006/bbrc.1999.0809.