PMID- 10364235
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 25
DP  - 1999 Jun 18
TI  - Human exonuclease 1 functionally complements its yeast homologues in DNA
      recombination, RNA primer removal, and mutation avoidance.
PG  - 17893-900
AB  - Yeast exonuclease 1 (Exo1) is induced during meiosis and plays an important role 
      in DNA homologous recombination and mismatch correction pathways. The human
      homolog, an 803-amino acid protein, shares 55% similarity to the yeast Exo1. In
      this report, we show that the enzyme functionally complements Saccharomyces
      cerevisiae Exo1 in recombination of direct repeat DNA fragments, UV resistance,
      and mutation avoidance by in vivo assays. Furthermore, the human enzyme
      suppresses the conditional lethality of a rad27Delta mutant, symptomatic of
      defective RNA primer removal. The purified recombinant enzyme not only displays
      5'-3' double strand DNA exonuclease activity, but also shows an RNase H activity.
      This result indicates a back-up function of exonuclease 1 to flap endonuclease-1 
      in RNA primer removal during lagging strand DNA synthesis.
FAU - Qiu, J
AU  - Qiu J
AD  - Department of Cell and Tumor Biology, City of Hope National Medical Center and
      Beckman Research Institute, Duarte, California 91010, USA.
FAU - Qian, Y
AU  - Qian Y
FAU - Chen, V
AU  - Chen V
FAU - Guan, M X
AU  - Guan MX
FAU - Shen, B
AU  - Shen B
LA  - eng
GR  - CA82468/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (RNA primers)
RN  - 0 (Recombinant Proteins)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 3.1.- (EXO1 protein, human)
RN  - EC 3.1.- (Exodeoxyribonucleases)
RN  - EC 3.1.11.1 (exodeoxyribonuclease I)
RN  - EC 3.1.26.4 (Ribonuclease H)
RN  - EC 6.5.1.- (DNA Repair Enzymes)
SB  - IM
MH  - Cell Division
MH  - Cycloheximide/pharmacology
MH  - DNA/biosynthesis
MH  - DNA Repair/genetics
MH  - DNA Repair Enzymes
MH  - Drug Resistance/genetics
MH  - Exodeoxyribonucleases/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Mutation/genetics
MH  - RNA/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - *Recombination, Genetic
MH  - Ribonuclease H/metabolism
MH  - Saccharomyces cerevisiae/*enzymology/genetics
MH  - Sequence Alignment
MH  - Substrate Specificity
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - 10.1074/jbc.274.25.17893 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 18;274(25):17893-900. doi: 10.1074/jbc.274.25.17893.