PMID- 10364231
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 25
DP  - 1999 Jun 18
TI  - PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase.
PG  - 17860-8
AB  - Poly(ADP-ribosylation) is a post-translational modification of nuclear proteins
      in response to DNA damage that activates the base excision repair machinery.
      Poly(ADP-ribose) polymerase which we will now call PARP-1, has been the only
      known enzyme of this type for over 30 years. Here, we describe a cDNA encoding a 
      62-kDa protein that shares considerable homology with the catalytic domain of
      PARP-1 and also contains a basic DNA-binding domain. We propose to call this
      enzyme poly(ADP-ribose) polymerase 2 (PARP-2). The PARP-2 gene maps to chromosome
      14C1 and 14q11.2 in mouse and human, respectively. Purified recombinant mouse
      PARP-2 is a damaged DNA-binding protein in vitro and catalyzes the formation of
      poly(ADP-ribose) polymers in a DNA-dependent manner. PARP-2 displays
      automodification properties similar to PARP-1. The protein is localized in the
      nucleus in vivo and may account for the residual poly(ADP-ribose) synthesis
      observed in PARP-1-deficient cells, treated with alkylating agents or hydrogen
      peroxide.
FAU - Ame, J C
AU  - Ame JC
AD  - UPR 9003 du CNRS, Ecole Superieure de Biotechnologie de Strasbourg, Boulevard
      Sebastien Brant, F-67400 Illkirch, France.
FAU - Rolli, V
AU  - Rolli V
FAU - Schreiber, V
AU  - Schreiber V
FAU - Niedergang, C
AU  - Niedergang C
FAU - Apiou, F
AU  - Apiou F
FAU - Decker, P
AU  - Decker P
FAU - Muller, S
AU  - Muller S
FAU - Hoger, T
AU  - Hoger T
FAU - Menissier-de Murcia, J
AU  - Menissier-de Murcia J
FAU - de Murcia, G
AU  - de Murcia G
LA  - eng
SI  - GENBANK/AJ007780
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Cloning, Molecular
MH  - DNA Damage/*genetics
MH  - DNA-Binding Proteins/chemistry/genetics
MH  - Enzyme Activation/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/enzymology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/metabolism
MH  - Poly(ADP-ribose) Polymerases/chemistry/*genetics
MH  - RNA, Messenger/metabolism
MH  - Recombinant Proteins/genetics
MH  - Sequence Alignment
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - 10.1074/jbc.274.25.17860 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 18;274(25):17860-8. doi: 10.1074/jbc.274.25.17860.