PMID- 10364228
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 25
DP  - 1999 Jun 18
TI  - Identification of the stef gene that encodes a novel guanine nucleotide exchange 
      factor specific for Rac1.
PG  - 17837-44
AB  - The Rho family GTPases are involved in a variety of cellular events by changing
      the organization of actin cytoskeletal networks in response to extracellular
      signals. However, it is not clearly known how their activities are spatially and 
      temporally regulated. Here we report the identification of a novel guanine
      nucleotide exchange factor for Rac1, STEF, which is related in overall amino acid
      sequence and modular structure to mouse Tiam1 and Drosophila SIF proteins. STEF
      protein contains two pleckstrin homology domains, a PDZ domain and a Dbl homology
      domain. The in vitro assay showed that STEF protein specifically enhanced the
      dissociation of GDP from Rac1 but not that from either RhoA or Cdc42. Expression 
      of a truncated STEF protein in culture cells induced membrane ruffling with
      altered actin localization, which implies that this protein also activates Rac1
      in vivo. The stef transcript was observed in restricted parts of mice, including 
      cartilaginous tissues and the cortical plate of the central nervous system during
      embryogenesis. These findings suggested that STEF protein participates in the
      control of cellular events in several developing tissues, possibly changing the
      actin cytoskeletal network by activating Rac1.
FAU - Hoshino, M
AU  - Hoshino M
AD  - Department of Molecular Genetics, National Institute of Neuroscience, NCNP, 4-1-1
      Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.
FAU - Sone, M
AU  - Sone M
FAU - Fukata, M
AU  - Fukata M
FAU - Kuroda, S
AU  - Kuroda S
FAU - Kaibuchi, K
AU  - Kaibuchi K
FAU - Nabeshima, Y
AU  - Nabeshima Y
FAU - Hama, C
AU  - Hama C
LA  - eng
SI  - GENBANK/AB022915
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein, Saccharomyces cerevisiae)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Cycle Proteins/metabolism
MH  - Cloning, Molecular
MH  - Fluorescein-5-isothiocyanate/metabolism
MH  - GTP-Binding Proteins/*genetics/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Guanine Nucleotide Exchange Factors
MH  - Guanosine Diphosphate/metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - Humans
MH  - KB Cells
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Proteins/chemistry/*genetics
MH  - RNA, Messenger/metabolism
MH  - Recombinant Proteins/genetics
MH  - Sequence Alignment
MH  - Transfection
MH  - cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
MH  - rac GTP-Binding Proteins
MH  - rhoA GTP-Binding Protein
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - 10.1074/jbc.274.25.17837 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 18;274(25):17837-44. doi: 10.1074/jbc.274.25.17837.