PMID- 10364222
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 25
DP  - 1999 Jun 18
TI  - The mechanism of adenosine formation in cells. Cloning of cytosolic
      5'-nucleotidase-I.
PG  - 17789-93
AB  - Adenosine increases blood flow and decreases excitatory nerve firing. In the
      heart, it reduces rate and force of contraction and preconditions the heart
      against injury by prolonged ischemia. Based on indirect kinetic arguments, an
      AMP-selective cytosolic 5'-nucleotidase designated cN-I has been implicated in
      adenosine formation during ATP breakdown. The molecular identity of cN-I is
      unknown, although an IMP/GMP-selective cytosolic 5'-nucleotidase (cN-II) and an
      ecto-5'-nucleotidase (e-N) have been cloned. We utilized the high abundance of
      cN-I in pigeon heart to purify a 40-kDa subunit for partial protein sequencing
      and subsequent cDNA cloning. We obtained a full-length clone encoding a novel
      40-kDa peptide, unrelated to cN-II or e-N, that was most abundant in heart,
      brain, and breast muscle. Immunolocalization in heart showed a striated
      cytoplasmic location, suggesting association with contractile elements. Transient
      expression in COS-7 cells, generated a 5'-nucleotidase that catalyzed adenosine
      formation from AMP, which was increased during ATP catabolism. In conclusion, the
      cloning and expression of cN-I provides definitive evidence of its ability to
      produce adenosine during ATP breakdown.
FAU - Sala-Newby, G B
AU  - Sala-Newby GB
AD  - University of Bristol, Bristol Heart Institute, Bristol BS2 8HW, United Kingdom.
FAU - Skladanowski, A C
AU  - Skladanowski AC
FAU - Newby, A C
AU  - Newby AC
LA  - eng
SI  - GENBANK/AJ131243
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 415SHH325A (Adenosine Monophosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.1.3.5 (5'-Nucleotidase)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - 5'-Nucleotidase/chemistry/*genetics
MH  - Adenosine/*biosynthesis
MH  - Adenosine Monophosphate/metabolism
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - COS Cells
MH  - Cloning, Molecular
MH  - Columbidae
MH  - Cytosol/enzymology
MH  - Immunohistochemistry
MH  - Molecular Sequence Data
MH  - Myocardium/*enzymology
MH  - RNA, Messenger/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Transfection
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - 10.1074/jbc.274.25.17789 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 18;274(25):17789-93. doi: 10.1074/jbc.274.25.17789.