PMID- 10364220
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 25
DP  - 1999 Jun 18
TI  - Structure and function of human prepro-orexin gene.
PG  - 17771-6
AB  - Orexin-A and -B are recently identified potent orexigenic peptides that are
      derived from the same precursor peptide and are highly specifically localized in 
      neurons located in the lateral hypothalamic area, a region classically implicated
      in feeding behavior. We cloned the whole length of the human prepro-orexin gene
      and corresponding cDNA. The human prepro-orexin mRNA was predicted to encode a
      131-residue precursor peptide (prepro-orexin). The human prepro-orexin gene
      consists of two exons and one intron distributed over 1432 base pairs. The
      143-base pair first exon includes the 5'-untranslated region and a small part of 
      the coding region that encodes the first seven residues of the secretory signal
      sequence. The second exon contains the remaining portion of the open reading
      frame and 3'-untranslated region. The 3.2 kilobase pairs of the 5'-upstream
      region from a cloned human prepro-orexin gene promoter is sufficient to direct
      the expression of the Escherichia coli beta-galactosidase (lacZ) gene in
      transgenic mice to neurons in the lateral hypothalamic area and adjacent regions.
      The lacZ-positive neurons were positively stained with anti-orexin antibody but
      not with anti-melanin-concentrating hormone antibody. These findings suggest that
      this genomic fragment contains all the necessary elements for appropriate
      expression of the gene and will be useful for the targeted expression of the
      exogenous gene in orexin-containing neurons. These mice might also be useful for 
      examining the molecular mechanisms by which orexin gene expression is regulated.
FAU - Sakurai, T
AU  - Sakurai T
AD  - Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki,
      305-8575, Japan. stakeshi@md.tsukuba.ac.jp
FAU - Moriguchi, T
AU  - Moriguchi T
FAU - Furuya, K
AU  - Furuya K
FAU - Kajiwara, N
AU  - Kajiwara N
FAU - Nakamura, T
AU  - Nakamura T
FAU - Yanagisawa, M
AU  - Yanagisawa M
FAU - Goto, K
AU  - Goto K
LA  - eng
SI  - GENBANK/AF118885
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (HCRT protein, human)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Orexins)
RN  - 0 (Protein Precursors)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/metabolism
MH  - Carrier Proteins/*genetics
MH  - Cloning, Molecular
MH  - Exons/genetics
MH  - Gene Expression Regulation
MH  - Genes, Reporter
MH  - Histocytochemistry
MH  - Humans
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Introns/genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/genetics
MH  - Neuropeptides/*genetics
MH  - Orexins
MH  - Protein Precursors/chemistry/*genetics
MH  - Protein Sorting Signals/genetics
MH  - RNA, Messenger/genetics
MH  - Recombinant Proteins
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - 10.1074/jbc.274.25.17771 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 18;274(25):17771-6. doi: 10.1074/jbc.274.25.17771.