PMID- 10364211
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 25
DP  - 1999 Jun 18
TI  - Molecular cloning and functional characterization of brefeldin A-ADP-ribosylated 
      substrate. A novel protein involved in the maintenance of the Golgi structure.
PG  - 17705-10
AB  - Brefeldin A (BFA) is a fungal metabolite that disassembles the Golgi apparatus
      into tubular networks and causes the dissociation of coatomer proteins from Golgi
      membranes. We have previously shown that an additional effect of BFA is to
      stimulate the ADP-ribosylation of two cytosolic proteins of 38 and 50 kDa
      (brefeldin A-ADP-riboslyated substrate (BARS)) and that this effect greatly
      facilitates the Golgi-disassembling activity of the toxin. In this study, BARS
      has been purified from rat brain cytosol and microsequenced, and the BARS cDNA
      has been cloned. BARS shares high homology with two known proteins,
      C-terminal-binding protein 1 (CtBP1) and CtBP2. It is therefore a third member of
      the CtBP family. The role of BARS in Golgi disassembly by BFA was verified in
      permeabilized cells. In the presence of dialyzed cytosol that had been previously
      depleted of BARS or treated with an anti-BARS antibody, BFA potently disassembled
      the Golgi. However, in cytosol complemented with purified BARS, or even in
      control cytosols containing physiological levels of BARS, the action of BFA on
      Golgi disassembly was strongly inhibited. These results suggest that BARS exerts 
      a negative control on Golgi tubulation, with important consequences for the
      structure and function of the Golgi complex.
FAU - Spano, S
AU  - Spano S
AD  - Istituto di Ricerche Farmacologiche "Mario Negri", Department of Cell Biology and
      Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro (Chieti), 20157
      Milano, Italy.
FAU - Silletta, M G
AU  - Silletta MG
FAU - Colanzi, A
AU  - Colanzi A
FAU - Alberti, S
AU  - Alberti S
FAU - Fiucci, G
AU  - Fiucci G
FAU - Valente, C
AU  - Valente C
FAU - Fusella, A
AU  - Fusella A
FAU - Salmona, M
AU  - Salmona M
FAU - Mironov, A
AU  - Mironov A
FAU - Luini, A
AU  - Luini A
FAU - Corda, D
AU  - Corda D
LA  - eng
SI  - GENBANK/AF067795
GR  - E.0982/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Ctbp1 protein, rat)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Transcription Factors)
RN  - 20350-15-6 (Brefeldin A)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.- (C-terminal binding protein)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factors)
SB  - IM
EIN - J Biol Chem 1999 Aug 27;274(35):25188. Spanfo S [corrected to Spano S]
MH  - ADP-Ribosylation Factors
MH  - Alcohol Oxidoreductases
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/metabolism
MH  - Brefeldin A/*pharmacology
MH  - COS Cells
MH  - Carrier Proteins/chemistry/*genetics
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/chemistry
MH  - GTP-Binding Proteins/*metabolism
MH  - Golgi Apparatus/*drug effects
MH  - Lung/metabolism
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/chemistry/genetics
MH  - Phosphoproteins/chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sequence Alignment
MH  - Spleen/metabolism
MH  - *Transcription Factors
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - 10.1074/jbc.274.25.17705 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 18;274(25):17705-10. doi: 10.1074/jbc.274.25.17705.