PMID- 10364207
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 25
DP  - 1999 Jun 18
TI  - Ded1p, a DEAD-box protein required for translation initiation in Saccharomyces
      cerevisiae, is an RNA helicase.
PG  - 17677-83
AB  - The Ded1 protein (Ded1p), a member of the DEAD-box family, has recently been
      shown to be essential for translation initiation in Saccharomyces cerevisiae.
      Here, we show that Ded1p purified from Escherichia coli has an ATPase activity,
      which is stimulated by various RNA substrates. Using an RNA strand-displacement
      assay, we show that Ded1p has also an ATP-dependent RNA unwinding activity.
      Hydrolysis of ATP is required for this activity: the replacement of ATP by a
      nonhydrolyzable analog or a mutation in the DEAD motif abolishing ATPase activity
      results in loss of RNA unwinding. We find that cells harboring a Ded1 protein
      with this mutated DEAD motif are nonviable, suggesting that the ATPase and RNA
      helicase activities of this protein are essential to the cell. Finally, RNA
      binding measurements indicate that the presence of ATP, but not ADP, increases
      the affinity of Ded1p for duplex versus single-stranded RNA; we discuss how this 
      differential effect might drive the unwinding reaction.
FAU - Iost, I
AU  - Iost I
AD  - Departement de Biochimie Medicale, Centre Medical Universitaire, Universite de
      Geneve, 1211 Geneva 4, Switzerland.
FAU - Dreyfus, M
AU  - Dreyfus M
FAU - Linder, P
AU  - Linder P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Nucleotides)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 63231-63-0 (RNA)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.- (DED1 protein, S cerevisiae)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
RN  - EC 3.6.4.13 (RNA Helicases)
SB  - IM
MH  - Adenosine Diphosphate/pharmacology
MH  - Adenosine Triphosphatases/metabolism
MH  - Adenosine Triphosphate/pharmacology
MH  - *Cell Cycle Proteins
MH  - Cell Survival
MH  - DEAD-box RNA Helicases
MH  - Enzyme Activation/drug effects
MH  - Escherichia coli
MH  - Fungal Proteins/genetics/*metabolism
MH  - Kinetics
MH  - Mutation
MH  - Nucleotides/pharmacology
MH  - Protein Biosynthesis
MH  - RNA/metabolism
MH  - RNA Helicases/genetics/*metabolism
MH  - RNA-Binding Proteins/genetics
MH  - Recombinant Proteins/genetics
MH  - Saccharomyces cerevisiae/*enzymology/genetics
MH  - *Saccharomyces cerevisiae Proteins
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - 10.1074/jbc.274.25.17677 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 18;274(25):17677-83. doi: 10.1074/jbc.274.25.17677.