PMID- 10364181
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 25
DP  - 1999 Jun 18
TI  - Evidence for a zinc uptake transporter in human prostate cancer cells which is
      regulated by prolactin and testosterone.
PG  - 17499-504
AB  - The glandular epithelial cells of the human prostate gland have the unique
      capability and function of accumulating the highest zinc levels of any soft
      tissue in the body. Zinc accumulation in the prostate is regulated by prolactin
      and testosterone; however, little information is available concerning the
      mechanisms associated with zinc accumulation and its regulation in prostate
      epithelial cells. In the present studies the uptake and accumulation of zinc were
      determined in the human malignant prostate cell lines LNCaP and PC-3. The results
      demonstrate that LNCaP cells and PC-3 cells possess the unique capability of
      accumulating high levels of zinc. Zinc accumulation in both cell types is
      stimulated by physiological concentrations of prolactin and testosterone. The
      studies reveal that these cells contain a rapid zinc uptake process indicative of
      a plasma membrane zinc transporter. Initial kinetic studies demonstrate that the 
      rapid uptake of zinc is effective under physiological conditions that reflect the
      total and mobile zinc levels in circulation. Correspondingly, genetic studies
      demonstrate the expression of a ZIP family zinc uptake transporter in both LNCaP 
      and PC-3 cells. The rapid zinc uptake transport process is stimulated by
      treatment of cells with physiological levels of prolactin and testosterone, which
      possibly is the result of the regulation of the ZIP-type zinc transporter gene.
      These zinc-accumulating characteristics are specific for prostate cells. The
      studies support the concept that these prostate cells express a unique
      hormone-responsive, plasma membrane-associated, rapid zinc uptake transporter
      gene associated with their unique ability to accumulate high zinc levels.
FAU - Costello, L C
AU  - Costello LC
AD  - Cellular and Molecular Biology Section, Department of Oral and Craniofacial
      Biological Sciences, University of Maryland Dental School, Baltimore, Maryland
      21201, USA. lcc001@dental.umaryland.edu
FAU - Liu, Y
AU  - Liu Y
FAU - Zou, J
AU  - Zou J
FAU - Franklin, R B
AU  - Franklin RB
LA  - eng
GR  - CA 71207/CA/NCI NIH HHS/United States
GR  - DK 28015/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Zinc Radioisotopes)
RN  - 3XMK78S47O (Testosterone)
RN  - 9002-62-4 (Prolactin)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Biological Transport/drug effects
MH  - Carrier Proteins/genetics/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Male
MH  - Prolactin/*pharmacology
MH  - Prostatic Neoplasms/*metabolism
MH  - Receptors, Androgen/genetics
MH  - Testosterone/*pharmacology
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Zinc/*metabolism
MH  - Zinc Radioisotopes
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - 10.1074/jbc.274.25.17499 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 18;274(25):17499-504. doi: 10.1074/jbc.274.25.17499.