PMID- 10363580
OWN - NLM
STAT- MEDLINE
DCOM- 19990707
LR  - 20190513
IS  - 0910-5050 (Print)
IS  - 0910-5050 (Linking)
VI  - 90
IP  - 4
DP  - 1999 Apr
TI  - Increased choline kinase activity and elevated phosphocholine levels in human
      colon cancer.
PG  - 419-24
AB  - Nuclear magnetic resonance spectroscopy has detected elevated phosphocholine
      levels in human tumor tissues and cells, and in cells that were transformed with 
      the activated Ha-ras gene and stimulated in vitro with growth-promoting factors
      such as platelet-derived growth factor, epidermal growth factor, and phorbol
      ester. However, the mechanism of the elevation and the function of the increased 
      phosphocholine levels have not been clearly demonstrated. We studied
      phosphocholine levels enzymatically and analyzed the activity of choline kinase, 
      which catalyzes the phosphorylation of choline to produce phosphocholine, in
      human colon cancer and adenoma. Both choline kinase activity and phosphocholine
      levels were increased in colon cancer and adenoma tissue. The activation of
      choline kinase and the increased levels of choline kinase alpha were partly
      responsible for the elevated phosphocholine levels. This study suggests that
      choline kinase might play a role in growth promotion or signal transduction in
      carcinogenesis.
FAU - Nakagami, K
AU  - Nakagami K
AD  - Second Department of Surgery, Gunma University School of Medicine, Maebashi.
FAU - Uchida, T
AU  - Uchida T
FAU - Ohwada, S
AU  - Ohwada S
FAU - Koibuchi, Y
AU  - Koibuchi Y
FAU - Suda, Y
AU  - Suda Y
FAU - Sekine, T
AU  - Sekine T
FAU - Morishita, Y
AU  - Morishita Y
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 0 (Protein Isoforms)
RN  - 107-73-3 (Phosphorylcholine)
RN  - EC 2.7.1.32 (Choline Kinase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blotting, Western
MH  - Case-Control Studies
MH  - Choline Kinase/*metabolism
MH  - Colonic Neoplasms/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Phosphorylcholine/*metabolism
MH  - Protein Isoforms/metabolism
PMC - PMC5926083
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - S0910505099800805 [pii]
AID - 10.1111/j.1349-7006.1999.tb00764.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 1999 Apr;90(4):419-24. doi: 10.1111/j.1349-7006.1999.tb00764.x.