PMID- 10362588
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20190115
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 276
IP  - 6
DP  - 1999 Jun
TI  - Molecular cloning and transmembrane structure of hCLCA2 from human lung, trachea,
      and mammary gland.
PG  - C1261-70
LID - 10.1152/ajpcell.1999.276.6.C1261 [doi]
AB  - The CLCA family of Ca2+-activated Cl- channels has recently been discovered, with
      an increasing number of closely related members isolated from different species. 
      Here we report the cloning of the second human homolog, hCLCA2, from a human lung
      cDNA library. Northern blot and RT-PCR analyses revealed additional expression in
      trachea and mammary gland. A primary translation product of 120 kDa was cleaved
      into two cell surface-associated glycoproteins of 86 and 34 kDa in transfected
      HEK-293 cells. hCLCA2 is the first CLCA homolog for which the transmembrane
      structure has been systematically studied. Glycosylation site scanning and
      protease protection assays revealed five transmembrane domains with a large,
      cysteine-rich, amino-terminal extracellular domain. Whole cell patch-clamp
      recordings of hCLCA2-transfected HEK-293 cells detected a slightly outwardly
      rectifying anion conductance that was increased in the presence of the Ca2+
      ionophore ionomycin and inhibited by DIDS, dithiothreitol, niflumic acid, and
      tamoxifen. Expression in human trachea and lung suggests that hCLCA2 may play a
      role in the complex pathogenesis of cystic fibrosis.
FAU - Gruber, A D
AU  - Gruber AD
AD  - Cancer Biology Laboratories, Department of Molecular Medicine, Cornell University
      College of Veterinary Medicine, Ithaca, New York 14853, USA.
FAU - Schreur, K D
AU  - Schreur KD
FAU - Ji, H L
AU  - Ji HL
FAU - Fuller, C M
AU  - Fuller CM
FAU - Pauli, B U
AU  - Pauli BU
LA  - eng
SI  - GENBANK/AF043977
GR  - CA-47668/CA/NCI NIH HHS/United States
GR  - CA-71626/CA/NCI NIH HHS/United States
GR  - DK-53090/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (CLCA2 protein, human)
RN  - 0 (Chloride Channels)
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Base Sequence/genetics
MH  - Breast/*metabolism
MH  - Cell Line
MH  - Chloride Channels/*chemistry/*genetics/metabolism/physiology
MH  - *Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Electrophysiology
MH  - Female
MH  - Humans
MH  - Lung/*metabolism
MH  - Molecular Sequence Data
MH  - Trachea/*metabolism
EDAT- 1999/06/11 00:00
MHDA- 1999/06/11 00:01
CRDT- 1999/06/11 00:00
PHST- 1999/06/11 00:00 [pubmed]
PHST- 1999/06/11 00:01 [medline]
PHST- 1999/06/11 00:00 [entrez]
AID - 10.1152/ajpcell.1999.276.6.C1261 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Jun;276(6):C1261-70. doi: 10.1152/ajpcell.1999.276.6.C1261.