PMID- 10362137
OWN - NLM
STAT- MEDLINE
DCOM- 19990624
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 81
IP  - 6
DP  - 1999 Jun 11
TI  - Establishment and characterization of 12 human colorectal-carcinoma cell lines.
PG  - 902-10
AB  - In this article, we describe the characteristics of 12 human colorectal-carcinoma
      cell lines established from 6 primary tumors and 6 metastatic sites of 11 Korean 
      colorectal-carcinoma patients, including the morphology in vivo and in vitro and 
      mutations of K-ras2, p15, p16, p53, APC, beta-catenin, hMLH1 and hMSH2 genes in
      vitro. No lines were contaminated with Mycoplasma or bacteria. All lines were
      proven to be unique by DNA-fingerprinting analysis. All lines expressed the
      surface carcino-embryonic antigen and secreted it into the supernatant fluid. The
      morphological correlation between the original tumors and cultured cells
      suggested that the original tumors showing mucinous adenocarcinoma correlated
      with floating aggregates in culture, and degree of desmoplasia in the original
      tumor correlated with attached growth in culture. Five of the cell lines showed
      mutations in the K-ras2 gene, and 6 of the cell lines showed mutations in the p53
      gene. The p15 gene was deleted in 2 cell lines, and the p16 gene was
      hypermethylated in 3 cell lines. The mutation of mismatch-repair genes (hMLH1 and
      hMSH2) was found in 4 lines, the APC gene and beta-catenin gene were mutated in 9
      and 2 lines respectively. These well-characterized colorectal-cancer cell lines
      should serve as useful tools for investigating the biological characteristics of 
      colorectal cancer.
FAU - Oh, J H
AU  - Oh JH
AD  - Laboratory of Cell Biology, Cancer Research Center and Cancer Research Institute,
      Korean Cell Line Bank, Seoul National University College of Medicine, Republic of
      Korea.
FAU - Ku, J L
AU  - Ku JL
FAU - Yoon, K A
AU  - Yoon KA
FAU - Kwon, H J
AU  - Kwon HJ
FAU - Kim, W H
AU  - Kim WH
FAU - Park, H S
AU  - Park HS
FAU - Yeo, K S
AU  - Yeo KS
FAU - Song, S Y
AU  - Song SY
FAU - Chung, J K
AU  - Chung JK
FAU - Park, J G
AU  - Park JG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CDKN2B protein, human)
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p15)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (MLH1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (beta Catenin)
RN  - EC 3.6.1.3 (MutL Protein Homolog 1)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Adult
MH  - Aged
MH  - Carrier Proteins
MH  - Cell Culture Techniques/*methods
MH  - *Cell Cycle Proteins
MH  - Colonic Neoplasms/genetics/pathology
MH  - Colorectal Neoplasms/*genetics/*pathology
MH  - Cyclin-Dependent Kinase Inhibitor p15
MH  - Cytoskeletal Proteins/genetics
MH  - DNA Repair
MH  - Female
MH  - Genes, APC
MH  - Genes, p16
MH  - Genes, p53
MH  - Genes, ras
MH  - Humans
MH  - Liver Neoplasms/genetics/pathology/secondary
MH  - Male
MH  - Middle Aged
MH  - MutL Protein Homolog 1
MH  - *Mutation
MH  - Neoplasm Metastasis
MH  - Neoplasm Proteins/genetics
MH  - Nuclear Proteins
MH  - Proteins/genetics
MH  - Rectal Neoplasms/genetics/pathology
MH  - *Trans-Activators
MH  - Transcription Factors/genetics
MH  - Tumor Cells, Cultured
MH  - *Tumor Suppressor Proteins
MH  - beta Catenin
EDAT- 1999/06/11 10:00
MHDA- 2000/06/20 09:00
CRDT- 1999/06/11 10:00
PHST- 1999/06/11 10:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/06/11 10:00 [entrez]
AID - 10.1002/(SICI)1097-0215(19990611)81:6<902::AID-IJC11>3.0.CO;2-T [pii]
AID - 10.1002/(sici)1097-0215(19990611)81:6<902::aid-ijc11>3.0.co;2-t [doi]
PST - ppublish
SO  - Int J Cancer. 1999 Jun 11;81(6):902-10. doi:
      10.1002/(sici)1097-0215(19990611)81:6<902::aid-ijc11>3.0.co;2-t.