PMID- 10360181
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20210507
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 5
DP  - 1999 May
TI  - A molecular mechanism for the phosphorylation-dependent regulation of
      heterotrimeric G proteins by phosducin.
PG  - 649-60
AB  - Visual signal transduction is a nearly noise-free process that is exquisitely
      well regulated over a wide dynamic range of light intensity. A key component in
      dark/light adaptation is phosducin, a phosphorylatable protein that modulates the
      amount of transducin heterotrimer (Gt alpha beta gamma) available through
      sequestration of the beta gamma subunits (Gt beta gamma). The structure of the
      phosphophosducin/Gt beta gamma complex combined with mutational and biophysical
      analysis provides a stereochemical mechanism for the regulation of the
      phosducin-Gt beta gamma interaction. Phosphorylation of serine 73 causes an
      order-to-disorder transition of a 20-residue stretch, including the
      phosphorylation site, by disrupting a helix-capping motif. This transition
      disrupts phosducin's interface with Gt beta gamma, leading to the release of
      unencumbered Gt beta gamma, which reassociates with the membrane and Gt alpha to 
      form a signaling-competent Gt alpha beta gamma heterotrimer.
FAU - Gaudet, R
AU  - Gaudet R
AD  - Department of Molecular Biophysics, Howard Hughes Medical Institute, Yale
      University, New Haven, Connecticut 06511, USA.
FAU - Savage, J R
AU  - Savage JR
FAU - McLaughlin, J N
AU  - McLaughlin JN
FAU - Willardson, B M
AU  - Willardson BM
FAU - Sigler, P B
AU  - Sigler PB
LA  - eng
SI  - PDB/1B9X
SI  - PDB/1B9Y
GR  - GM22324/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (Eye Proteins)
RN  - 0 (GTP-Binding Protein Regulators)
RN  - 0 (Phosphoproteins)
RN  - 0 (phosducin)
RN  - 452VLY9402 (Serine)
RN  - 9009-81-8 (Rhodopsin)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
SB  - S
MH  - Animals
MH  - Circular Dichroism
MH  - DNA Mutational Analysis
MH  - Endopeptidases/metabolism
MH  - Eye Proteins/chemistry/*genetics/metabolism
MH  - GTP-Binding Protein Regulators
MH  - GTP-Binding Proteins/*chemistry/*metabolism
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Sequence Data
MH  - Mutagenesis/physiology
MH  - Phosphoproteins/chemistry/*genetics/metabolism
MH  - Phosphorylation
MH  - Protein Kinases/metabolism
MH  - Protein Structure, Secondary
MH  - Protein Structure, Tertiary
MH  - Rats
MH  - Rhodopsin/metabolism
MH  - Serine
MH  - Vision, Ocular/*physiology
EDAT- 1999/06/09 00:00
MHDA- 1999/06/09 00:01
CRDT- 1999/06/09 00:00
PHST- 1999/06/09 00:00 [pubmed]
PHST- 1999/06/09 00:01 [medline]
PHST- 1999/06/09 00:00 [entrez]
AID - S1097-2765(00)80358-5 [pii]
AID - 10.1016/s1097-2765(00)80358-5 [doi]
PST - ppublish
SO  - Mol Cell. 1999 May;3(5):649-60. doi: 10.1016/s1097-2765(00)80358-5.