PMID- 10359840
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 12
DP  - 1999 Jun 8
TI  - Isolation and characterization of a dual-substrate phosphodiesterase gene family:
      PDE10A.
PG  - 7071-6
AB  - We report here the cloning, expression, and characterization of a dual-substrate,
      cAMP and cGMP, cyclic nucleotide phosphodiesterase (PDE) from mouse. This PDE
      contains the consensus sequence for a PDE catalytic domain, but shares <50%
      sequence identity with the catalytic domains of all other known PDEs and,
      therefore, represents a new PDE gene family, designated PDE10A. The cDNA for
      PDE10A is 3, 370 nt in length. It includes a full ORF, contains three in-frame
      stop codons upstream of the first methionine, and is predicted to encode a 779-aa
      enzyme. At the N terminus PDE10A has two GAF domains homologous to many signaling
      molecules, including PDE2, PDE5, and PDE6, which likely constitute a low-affinity
      binding site for cGMP. PDE10A hydrolyzes cAMP with a Km of 0.05 microM and cGMP
      with a Km of 3 microM. Although PDE10A has a lower Km for cAMP, the Vmax ratio
      (cGMP/cAMP) is 4.7. RNA distribution studies indicate that PDE10A is expressed at
      highest levels in testis and brain.
FAU - Soderling, S H
AU  - Soderling SH
AD  - Department of Pharmacology, Box 357280, University of Washington, Seattle, WA
      98195, USA.
FAU - Bayuga, S J
AU  - Bayuga SJ
FAU - Beavo, J A
AU  - Beavo JA
LA  - eng
SI  - GENBANK/AF110507
GR  - R01 DK021723/DK/NIDDK NIH HHS/United States
GR  - T32 GM007750/GM/NIGMS NIH HHS/United States
GR  - DK21723/DK/NIDDK NIH HHS/United States
GR  - GM07750/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 3.1.4.- (Pde10a protein, mouse)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - EC 3.1.4.17 (3',5'-Cyclic-AMP Phosphodiesterases)
RN  - EC 3.1.4.35 (3',5'-Cyclic-GMP Phosphodiesterases)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - 3',5'-Cyclic-AMP Phosphodiesterases/*genetics/metabolism
MH  - 3',5'-Cyclic-GMP Phosphodiesterases/*genetics/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Cyclic AMP/metabolism
MH  - Cyclic GMP/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphoric Diester Hydrolases/*genetics/metabolism
MH  - Sequence Alignment
MH  - Substrate Specificity
PMC - PMC22059
EDAT- 1999/06/09 00:00
MHDA- 1999/06/09 00:01
CRDT- 1999/06/09 00:00
PHST- 1999/06/09 00:00 [pubmed]
PHST- 1999/06/09 00:01 [medline]
PHST- 1999/06/09 00:00 [entrez]
AID - 10.1073/pnas.96.12.7071 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):7071-6. doi: 10.1073/pnas.96.12.7071.