PMID- 10359786
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 12
DP  - 1999 Jun 8
TI  - Two distinct forms of the 64,000 Mr protein of the cleavage stimulation factor
      are expressed in mouse male germ cells.
PG  - 6763-8
AB  - Polyadenylation in male germ cells differs from that in somatic cells. Many germ 
      cell mRNAs do not contain the canonical AAUAAA in their 3' ends but are
      efficiently polyadenylated. To determine whether the 64,000 Mr protein of the
      cleavage stimulation factor (CstF-64) is altered in male germ cells, we examined 
      its expression in mouse testis. In addition to the 64,000 Mr form, we found a
      related approximately 70,000 Mr protein that is abundant in testis, at low levels
      in brain, and undetectable in all other tissues examined. Expression of the
      approximately 70,000 Mr CstF-64 was limited to meiotic spermatocytes and
      postmeiotic spermatids in testis. In contrast, the 64,000 Mr form was absent from
      spermatocytes, suggesting that the testis-specific CstF-64 might control
      expression of meiosis-specific genes. To determine why the 64,000 Mr CstF-64 is
      not expressed in spermatocytes, we mapped its chromosomal location to the X
      chromosome in both mouse and human. CstF-64 may, therefore, be absent in
      spermatocytes because the X chromosome is inactivated during male meiosis. By
      extension, the testis-specific CstF-64 may be expressed from an autosomal homolog
      of the X chromosomal gene.
FAU - Wallace, A M
AU  - Wallace AM
AD  - Department of Cell Biology and Biochemistry, Texas Tech University Health
      Sciences Center, Lubbock, TX 79430, USA.
FAU - Dass, B
AU  - Dass B
FAU - Ravnik, S E
AU  - Ravnik SE
FAU - Tonk, V
AU  - Tonk V
FAU - Jenkins, N A
AU  - Jenkins NA
FAU - Gilbert, D J
AU  - Gilbert DJ
FAU - Copeland, N G
AU  - Copeland NG
FAU - MacDonald, C C
AU  - MacDonald CC
LA  - eng
GR  - R01 HD037109/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (mRNA Cleavage and Polyadenylation Factors)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Isoforms/biosynthesis/genetics
MH  - RNA-Binding Proteins/*biosynthesis/genetics
MH  - Spermatids/*metabolism
MH  - Testis/*metabolism
MH  - mRNA Cleavage and Polyadenylation Factors
PMC - PMC21989
EDAT- 1999/06/09 00:00
MHDA- 1999/06/09 00:01
CRDT- 1999/06/09 00:00
PHST- 1999/06/09 00:00 [pubmed]
PHST- 1999/06/09 00:01 [medline]
PHST- 1999/06/09 00:00 [entrez]
AID - 10.1073/pnas.96.12.6763 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6763-8. doi: 10.1073/pnas.96.12.6763.