PMID- 10359768
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 12
DP  - 1999 Jun 8
TI  - CPF: an orphan nuclear receptor that regulates liver-specific expression of the
      human cholesterol 7alpha-hydroxylase gene.
PG  - 6660-5
AB  - Cholesterol 7alpha-hydroxylase is the first and rate-limiting enzyme in a pathway
      through which cholesterol is metabolized to bile acids. The gene encoding
      cholesterol 7alpha-hydroxylase, CYP7A, is expressed exclusively in the liver.
      Overexpression of CYP7A in hamsters results in a reduction of serum cholesterol
      levels, suggesting that the enzyme plays a central role in cholesterol
      homeostasis. Here, we report the identification of a hepatic-specific
      transcription factor that binds to the promoter of the human CYP7A gene. We
      designate this factor CPF, for CYP7A promoter binding factor. Mutation of the CPF
      binding site within the CYP7A promoter abolished hepatic-specific expression of
      the gene in transient transfection assays. A cDNA encoding CPF was cloned and
      identified as a human homolog of the Drosophila orphan nuclear receptor fushi
      tarazu F1 (Ftz-F1). Cotransfection of a CPF expression plasmid and a CYP7A
      reporter gene resulted in specific induction of CYP7A-directed transcription.
      These observations suggest that CPF is a key regulator of human CYP7A gene
      expression in the liver.
FAU - Nitta, M
AU  - Nitta M
AD  - Biology Department, Tularik Inc., Two Corporate Drive, South San Francisco, CA
      94080, USA.
FAU - Ku, S
AU  - Ku S
FAU - Brown, C
AU  - Brown C
FAU - Okamoto, A Y
AU  - Okamoto AY
FAU - Shan, B
AU  - Shan B
LA  - eng
SI  - GENBANK/AF146343
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Transcription Factors)
RN  - EC 1.14.14.23 (Cholesterol 7-alpha-Hydroxylase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cholesterol 7-alpha-Hydroxylase/*biosynthesis/genetics
MH  - Cricetinae
MH  - *Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Liver/*enzymology
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Promoter Regions, Genetic
MH  - Sequence Alignment
MH  - Transcription Factors/*genetics
MH  - Transfection
PMC - PMC21971
EDAT- 1999/06/09 00:00
MHDA- 1999/06/09 00:01
CRDT- 1999/06/09 00:00
PHST- 1999/06/09 00:00 [pubmed]
PHST- 1999/06/09 00:01 [medline]
PHST- 1999/06/09 00:00 [entrez]
AID - 10.1073/pnas.96.12.6660 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6660-5. doi: 10.1073/pnas.96.12.6660.