PMID- 10359671
OWN - NLM
STAT- MEDLINE
DCOM- 19990802
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 340 ( Pt 3)
DP  - 1999 Jun 15
TI  - Cloning and characterization of a novel human olfactory
      UDP-glucuronosyltransferase.
PG  - 837-43
AB  - Xenobiotic metabolizing enzymes in the olfactory epithelium have been suggested
      to catalyse inactivation and facilitate elimination of odorants. We report here
      the molecular cloning and functional characterization of a human olfactory
      UDP-glucuronosyltransferase (UGT). The cloned protein is composed of 527 amino
      acids with an identity of 87% with a rat olfactory UGT and of 43-62% with other
      human UGT isoforms. Based on the sequence homology, it has been designated
      hUGT2A1. The gene was mapped to chromosome 4q13 by fluorescence in situ
      hybridization. The expression appeared to be specific for the olfactory tissue.
      The substrate specificity of this isoform was assessed using Chinese hamster V79 
      cells stably transfected with the isolated cDNA. The expressed enzyme showed a
      broad substrate spectrum including a range of phenolic compounds as well as
      aliphatic and monoterpenoid alcohols, among them many odorants. Furthermore, some
      steroids, especially androgens, some drugs and carcinogens were conjugated. The
      results support a role of the enzyme in olfactory perception and in protection of
      the neural system against airborne hazardous chemicals.
FAU - Jedlitschky, G
AU  - Jedlitschky G
AD  - Department of Molecular and Cellular Pathology, Ninewells Hospital and Medical
      School, University of Dundee, Dundee DD1 9SY, Scotland, UK.
FAU - Cassidy, A J
AU  - Cassidy AJ
FAU - Sales, M
AU  - Sales M
FAU - Pratt, N
AU  - Pratt N
FAU - Burchell, B
AU  - Burchell B
LA  - eng
SI  - GENBANK/AJ006054
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Alcohols)
RN  - 0 (Isoenzymes)
RN  - 0 (Phenols)
RN  - 0 (RNA, Messenger)
RN  - 0 (Steroids)
RN  - EC 2.4.1.17 (Glucuronosyltransferase)
SB  - IM
MH  - Alcohols/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chromosomes, Human, Pair 4/genetics
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Cricetulus
MH  - Glucuronosyltransferase/chemistry/*genetics/metabolism
MH  - Humans
MH  - Isoenzymes/chemistry/genetics
MH  - Molecular Sequence Data
MH  - Olfactory Bulb/*enzymology
MH  - Organ Specificity
MH  - Phenols/metabolism
MH  - Phylogeny
MH  - RNA, Messenger/analysis/genetics/metabolism
MH  - Rats
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Steroids/metabolism
MH  - Substrate Specificity
MH  - Transfection
PMC - PMC1220318
EDAT- 1999/06/09 00:00
MHDA- 1999/06/09 00:01
CRDT- 1999/06/09 00:00
PHST- 1999/06/09 00:00 [pubmed]
PHST- 1999/06/09 00:01 [medline]
PHST- 1999/06/09 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Jun 15;340 ( Pt 3):837-43.