PMID- 10359651
OWN - NLM
STAT- MEDLINE
DCOM- 19990802
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 340 ( Pt 3)
DP  - 1999 Jun 15
TI  - Lipid phosphate phosphohydrolase-1 degrades exogenous glycerolipid and
      sphingolipid phosphate esters.
PG  - 677-86
AB  - Lipid phosphate phosphohydrolase (LPP)-1 cDNA was cloned from a rat liver cDNA
      library. It codes for a 32-kDa protein that shares 87 and 82% amino acid sequence
      identities with putative products of murine and human LPP-1 cDNAs, respectively. 
      Membrane fractions of rat2 fibroblasts that stably expressed mouse or rat LPP-1
      exhibited 3.1-3. 6-fold higher specific activities for phosphatidate
      dephosphorylation compared with vector controls. Increases in the
      dephosphorylation of lysophosphatidate, ceramide 1-phosphate, sphingosine
      1-phosphate and diacylglycerol pyrophosphate were similar to those for
      phosphatidate. Rat2 fibroblasts expressing mouse LPP-1 cDNA showed 1.6-2.3-fold
      increases in the hydrolysis of exogenous lysophosphatidate, phosphatidate and
      ceramide 1-phosphate compared with vector control cells. Recombinant LPP-1 was
      located partially in plasma membranes with its C-terminus on the cytosolic
      surface. Lysophosphatidate dephosphorylation was inhibited by extracellular Ca2+ 
      and this inhibition was diminished by extracellular Mg2+. Changing intracellular 
      Ca2+ concentrations did not alter exogenous lysophosphatidate dephosphorylation
      significantly. Permeabilized fibroblasts showed relatively little latency for the
      dephosphorylation of exogenous lysophosphatidate. LPP-1 expression decreased the 
      activation of mitogen-activated protein kinase and DNA synthesis by exogenous
      lysophosphatidate. The product of LPP-1 cDNA is concluded to act partly to
      degrade exogenous lysophosphatidate and thereby regulate its effects on cell
      signalling.
FAU - Jasinska, R
AU  - Jasinska R
AD  - Department of Biochemistry (Signal Transduction Laboratories), Lipid and
      Lipoprotein Research Group, University of Alberta, 357 Heritage Medical Research 
      Centre, Edmonton, Alberta, T6G 2S2, Canada.
FAU - Zhang, Q X
AU  - Zhang QX
FAU - Pilquil, C
AU  - Pilquil C
FAU - Singh, I
AU  - Singh I
FAU - Xu, J
AU  - Xu J
FAU - Dewald, J
AU  - Dewald J
FAU - Dillon, D A
AU  - Dillon DA
FAU - Berthiaume, L G
AU  - Berthiaume LG
FAU - Carman, G M
AU  - Carman GM
FAU - Waggoner, D W
AU  - Waggoner DW
FAU - Brindley, D N
AU  - Brindley DN
LA  - eng
GR  - R01 GM028140/GM/NIGMS NIH HHS/United States
GR  - GM-28140/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Esters)
RN  - 0 (Glycolipids)
RN  - 0 (Lysophospholipids)
RN  - 0 (Phosphates)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Sphingolipids)
RN  - 0 (glycerolglycolipids)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 3.1.3.- (lipid phosphate phosphatase)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.1.3.4 (Phosphatidate Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/pharmacology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Cell Line
MH  - Cell Membrane/enzymology
MH  - Cell Membrane Permeability
MH  - DNA/biosynthesis
MH  - Enzyme Activation/drug effects
MH  - Esters/*metabolism
MH  - Glycolipids/*metabolism
MH  - Humans
MH  - Hydrolysis/drug effects
MH  - Kinetics
MH  - Lysophospholipids/metabolism/pharmacology
MH  - Mice
MH  - Phosphates/*metabolism
MH  - Phosphatidate Phosphatase/chemistry/genetics/*metabolism
MH  - Phosphoric Monoester Hydrolases/chemistry/genetics/*metabolism
MH  - Phosphorylation/drug effects
MH  - Rats
MH  - Recombinant Fusion Proteins/analysis/biosynthesis/metabolism
MH  - Sphingolipids/*metabolism
MH  - Substrate Specificity
PMC - PMC1220298
EDAT- 1999/06/09 00:00
MHDA- 1999/06/09 00:01
CRDT- 1999/06/09 00:00
PHST- 1999/06/09 00:00 [pubmed]
PHST- 1999/06/09 00:01 [medline]
PHST- 1999/06/09 00:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Jun 15;340 ( Pt 3):677-86.