PMID- 10359595
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 10
IP  - 6
DP  - 1999 Jun
TI  - Membrane tubule-mediated reassembly and maintenance of the Golgi complex is
      disrupted by phospholipase A2 antagonists.
PG  - 1763-82
AB  - Although membrane tubules can be found extending from, and associated with, the
      Golgi complex of eukaryotic cells, their physiological function has remained
      unclear. To gain insight into the biological significance of membrane tubules, we
      have developed methods for selectively preventing their formation. We show here
      that a broad range of phospholipase A2 (PLA2) antagonists not only arrest
      membrane tubule-mediated events that occur late in the assembly of the Golgi
      complex but also perturb its normal steady-state tubulovesicular architecture by 
      inducing a reversible fragmentation into separate "mini-stacks." In addition, we 
      show that these same compounds prevent the formation of membrane tubules from
      Golgi stacks in an in vitro reconstitution system. This in vitro assay was
      further used to demonstrate that the relevant PLA2 activity originates from the
      cytoplasm. Taken together, these results demonstrate that Golgi membrane tubules,
      sensitive to potent and selective PLA2 antagonists, mediate both late events in
      the reassembly of the Golgi complex and the dynamic maintenance of its
      steady-state architecture. In addition, they implicate a role for cytoplasmic
      PLA2 enzymes in mediating these membrane trafficking events.
FAU - de Figueiredo, P
AU  - de Figueiredo P
AD  - Section of Biochemistry, Molecular, and Cell Biology, Cornell University, Ithaca,
      New York 14853, USA.
FAU - Polizotto, R S
AU  - Polizotto RS
FAU - Drecktrah, D
AU  - Drecktrah D
FAU - Brown, W J
AU  - Brown WJ
LA  - eng
GR  - R01 DK051596/DK/NIDDK NIH HHS/United States
GR  - R56 DK051596/DK/NIDDK NIH HHS/United States
GR  - DK-51596/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Aminobenzoates)
RN  - 0 (Chlorobenzoates)
RN  - 0 (Cinnamates)
RN  - 0 (Coatomer Protein)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Quinones)
RN  - 0 (ortho-Aminobenzoates)
RN  - 20350-15-6 (Brefeldin A)
RN  - 71678-03-0 (illimaquinone)
RN  - 99754-06-0 (2-(4-amylcinnamoyl)amino-4-chlorobenzoic acid)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
SB  - IM
MH  - Aminobenzoates/pharmacology
MH  - Animals
MH  - Brefeldin A/pharmacology
MH  - Cell-Free System
MH  - Cells, Cultured
MH  - Chlorobenzoates
MH  - Cinnamates/pharmacology
MH  - Coatomer Protein
MH  - Endoplasmic Reticulum/metabolism
MH  - Enzyme Inhibitors/*pharmacology
MH  - Golgi Apparatus/drug effects/*metabolism/virology
MH  - Intracellular Membranes/drug effects/*ultrastructure
MH  - Liver/cytology/drug effects
MH  - Membrane Proteins/drug effects/metabolism
MH  - Microtubules/drug effects/metabolism/*ultrastructure
MH  - Phospholipases A/*antagonists & inhibitors
MH  - Phospholipases A2
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Quinones/pharmacology
MH  - Rats
MH  - Vesicular stomatitis Indiana virus/metabolism
MH  - ortho-Aminobenzoates
PMC - PMC25369
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
AID - 10.1091/mbc.10.6.1763 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1999 Jun;10(6):1763-82. doi: 10.1091/mbc.10.6.1763.