PMID- 10359581
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 189
IP  - 11
DP  - 1999 Jun 7
TI  - MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like
      receptor 4.
PG  - 1777-82
AB  - Toll-like receptor 4 (TLR4) is a mammalian homologue of Drosophila Toll, a
      leucine-rich repeat molecule that can trigger innate responses against pathogens.
      The TLR4 gene has recently been shown to be mutated in C3H/HeJ and C57BL/10ScCr
      mice, both of which are low responders to lipopolysaccharide (LPS). TLR4 may be a
      long-sought receptor for LPS. However, transfection of TLR4 does not confer LPS
      responsiveness on a recipient cell line, suggesting a requirement for an
      additional molecule. Here, we report that a novel molecule, MD-2, is requisite
      for LPS signaling of TLR4. MD-2 is physically associated with TLR4 on the cell
      surface and confers responsiveness to LPS. MD-2 is thus a link between TLR4 and
      LPS signaling. Identification of this new receptor complex has potential
      implications for understanding host defense, as well as pathophysiologic,
      mechanisms.
FAU - Shimazu, R
AU  - Shimazu R
AD  - Department of Immunology, Saga Medical School, Saga, Japan.
FAU - Akashi, S
AU  - Akashi S
FAU - Ogata, H
AU  - Ogata H
FAU - Nagai, Y
AU  - Nagai Y
FAU - Fukudome, K
AU  - Fukudome K
FAU - Miyake, K
AU  - Miyake K
FAU - Kimoto, M
AU  - Kimoto M
LA  - eng
SI  - GENBANK/AB018549
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, Surface)
RN  - 0 (DNA, Complementary)
RN  - 0 (Drosophila Proteins)
RN  - 0 (LY96 protein, human)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Lymphocyte Antigen 96)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/genetics/*immunology
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Complementary/genetics
MH  - *Drosophila Proteins
MH  - Humans
MH  - Lipopolysaccharides/*pharmacology
MH  - Lymphocyte Antigen 96
MH  - Membrane Glycoproteins/genetics/*immunology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - NF-kappa B/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Cell Surface/genetics/*immunology
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
MH  - Transfection
PMC - PMC2193086
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
AID - 10.1084/jem.189.11.1777 [doi]
PST - ppublish
SO  - J Exp Med. 1999 Jun 7;189(11):1777-82. doi: 10.1084/jem.189.11.1777.