PMID- 10359081
OWN - NLM
STAT- MEDLINE
DCOM- 19990722
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 450
IP  - 3
DP  - 1999 May 7
TI  - The HIV-1 Vpr co-activator induces a conformational change in TFIIB.
PG  - 235-9
AB  - Vpr is a HIV-1 virion-associated protein which plays a role in viral replication 
      and in transcription and cell proliferation. We have previously reported that Vpr
      stimulates transcription of genes lacking a common DNA target sequence likely
      through its ability to interact with TFIIB. However, the molecular mechanism of
      the Vpr-mediated transcription remains to be precisely defined. In this in vitro 
      study, we show that the binding site of Vpr in TFIIB overlaps the domain of TFIIB
      which is engaged in the intramolecular bridge between the N- and C-terminus of
      TFIIB, highly suggesting that binding of Vpr may induce a change in the
      conformation of TFIIB. Indeed, with a partial proteolysis assay using V8
      protease, we demonstrate that Vpr has the ability to change the conformation of
      TFIIB. We investigated in this partial proteolysis assay a series of Vpr-mutated 
      proteins previously defined for their transactivation properties. Our data show a
      correlation between the ability of Vpr-mutated proteins to stimulate
      transcription and their ability to induce a conformational change in TFIIB,
      indicating a functional relevance of the Vpr-TFIIB interaction.
FAU - Agostini, I
AU  - Agostini I
AD  - INSERM U372, Marseille, France.
FAU - Navarro, J M
AU  - Navarro JM
FAU - Bouhamdan, M
AU  - Bouhamdan M
FAU - Willetts, K
AU  - Willetts K
FAU - Rey, F
AU  - Rey F
FAU - Spire, B
AU  - Spire B
FAU - Vigne, R
AU  - Vigne R
FAU - Pomerantz, R
AU  - Pomerantz R
FAU - Sire, J
AU  - Sire J
LA  - eng
GR  - 38666/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Gene Products, vpr)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factor TFIIB)
RN  - 0 (Transcription Factors)
RN  - 0 (vpr Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - Binding Sites
MH  - Gene Products, vpr/genetics/*metabolism
MH  - HIV-1/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Protein Conformation
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Transcription Factor TFIIB
MH  - Transcription Factors/*chemistry/*metabolism
MH  - vpr Gene Products, Human Immunodeficiency Virus
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
AID - S0014-5793(99)00501-3 [pii]
AID - 10.1016/s0014-5793(99)00501-3 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 May 7;450(3):235-9. doi: 10.1016/s0014-5793(99)00501-3.