PMID- 10359073
OWN - NLM
STAT- MEDLINE
DCOM- 19990722
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 450
IP  - 3
DP  - 1999 May 7
TI  - Identification and cloning of TWIK-originated similarity sequence (TOSS): a novel
      human 2-pore K+ channel principal subunit.
PG  - 191-6
AB  - We have identified and cloned a new member of the mammalian tandem pore domain K+
      channel subunit family, TWIK-originated similarity sequence, from a human testis 
      cDNA library. The 939 bp open reading frame encodes a 313 amino acid polypeptide 
      with a calculated Mr of 33.7 kDa. Despite the same predicted topology, there is a
      relatively low sequence homology between TWIK-originated similarity sequence and 
      other members of the mammalian tandem pore domain K+ channel subunit family
      group. TWIK-originated similarity sequence shares a low (< 30%) identity with the
      other mammalian tandem pore domain K+ channel subunit family group members and
      the highest identity (34%) with TWIK-1 at the amino acid level. Similar low
      levels of sequence homology exist between all members of the mammalian tandem
      pore domain K+ channel subunit family. Potential glycosylation and consensus PKC 
      sites are present. Northern analysis revealed species and tissue-specific
      expression patterns. Expression of TWIK-originated similarity sequence is
      restricted to human pancreas, placenta and heart, while in the mouse,
      TWIK-originated similarity sequence is expressed in the liver. No functional
      currents were observed in Xenopus laevis oocytes or HEK293T cells, suggesting
      that TWIK-originated similarity sequence may be targeted to locations other than 
      the plasma membrane or that TWIK-originated similarity sequence may represent a
      novel regulatory mammalian tandem pore domain K+ channel subunit family subunit.
FAU - Pountney, D J
AU  - Pountney DJ
AD  - Department of Pediatric Cardiology, New York University School of Medicine, NY
      10016, USA. david.pountney@med.nyu.edu
FAU - Gulkarov, I
AU  - Gulkarov I
FAU - Vega-Saenz de Miera, E
AU  - Vega-Saenz de Miera E
FAU - Holmes, D
AU  - Holmes D
FAU - Saganich, M
AU  - Saganich M
FAU - Rudy, B
AU  - Rudy B
FAU - Artman, M
AU  - Artman M
FAU - Coetzee, W A
AU  - Coetzee WA
LA  - eng
SI  - GENBANK/AF134149
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (KCNK1 protein, human)
RN  - 0 (Kcnk1 protein, mouse)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Tandem Pore Domain)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Humans
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Potassium Channels/*genetics
MH  - *Potassium Channels, Tandem Pore Domain
MH  - RNA, Messenger
MH  - Sequence Homology, Amino Acid
MH  - Testis/*metabolism
MH  - Xenopus laevis
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
AID - S0014-5793(99)00495-0 [pii]
AID - 10.1016/s0014-5793(99)00495-0 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 May 7;450(3):191-6. doi: 10.1016/s0014-5793(99)00495-0.