PMID- 10358095
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 24
DP  - 1999 Jun 11
TI  - p300/cAMP-responsive element-binding protein interactions with ets-1 and ets-2 in
      the transcriptional activation of the human stromelysin promoter.
PG  - 17342-52
AB  - In this paper we show that transcription factors Ets-1 and Ets-2 recruit
      transcription adapter proteins p300 and CBP (cAMP-responsive element-binding
      protein) during the transcriptional activation of the human stromelysin promoter,
      which contains palindromic Ets-binding sites. Ets-2 and p300/CBP exist as a
      complex in vivo. Two regions of p300/CBP between amino acids (a.a.) 328 and 596
      and a. a. 1678 and 2370 independently can interact with Ets-1 and Ets-2 in vitro 
      and in vivo. Both these regions of p300/CBP bind to the transactivation domain of
      Ets-2, whereas the C-terminal region binds only to the DNA binding domain of
      Ets-2. The N- and the C-terminal regions of CBP (a.a. 1-1097 and 1678-2442,
      respectively) which lack histone acetylation activity independently are capable
      of coactivating Ets-2. Other Ets family transcription factors failed to cooperate
      with p300/CBP in stimulating the stromelysin promoter. The LXXLL sequence,
      reported to be important in receptor-coactivator interactions, does not appear to
      play a role in the interaction of Ets-2 with p300/CBP. Previous studies have
      shown that the stimulation of transcriptional activation activity of Ets-2
      requires phosphorylation of threonine 72 by the Ras/mitogen-activated protein
      kinase signaling pathway. We show that mutation of this site does not affect its 
      capacity to bind to and to cooperate with p300/CBP.
FAU - Jayaraman, G
AU  - Jayaraman G
AD  - Lurie Cancer Center and Microbiology and Immunology Department, Northwestern
      University Medical School, Chicago, Illinois 60611, USA.
FAU - Srinivas, R
AU  - Srinivas R
FAU - Duggan, C
AU  - Duggan C
FAU - Ferreira, E
AU  - Ferreira E
FAU - Swaminathan, S
AU  - Swaminathan S
FAU - Somasundaram, K
AU  - Somasundaram K
FAU - Williams, J
AU  - Williams J
FAU - Hauser, C
AU  - Hauser C
FAU - Kurkinen, M
AU  - Kurkinen M
FAU - Dhar, R
AU  - Dhar R
FAU - Weitzman, S
AU  - Weitzman S
FAU - Buttice, G
AU  - Buttice G
FAU - Thimmapaya, B
AU  - Thimmapaya B
LA  - eng
GR  - AG11536/AG/NIA NIH HHS/United States
GR  - CA74403/CA/NCI NIH HHS/United States
GR  - NCI 1P20CA65764/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ERF protein, human)
RN  - 0 (ERG protein, human)
RN  - 0 (ETS1 protein, human)
RN  - 0 (ETS2 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Protein c-ets-1)
RN  - 0 (Proto-Oncogene Protein c-ets-2)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcriptional Regulator ERG)
RN  - 0 (transcription factor PEA3)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (CREBBP protein, human)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA3 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 3)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Binding Sites
MH  - CREB-Binding Protein
MH  - Calcium-Calmodulin-Dependent Protein Kinases/genetics/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Matrix Metalloproteinase 3/*biosynthesis/genetics
MH  - Mutation
MH  - Nuclear Proteins/*metabolism
MH  - Nuclear Receptor Coactivator 3
MH  - Precipitin Tests
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Proto-Oncogene Protein c-ets-1
MH  - Proto-Oncogene Protein c-ets-2
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-ets
MH  - *Repressor Proteins
MH  - Trans-Activators/genetics/*metabolism
MH  - Transcription Factors/*metabolism
MH  - *Transcriptional Activation
MH  - Transcriptional Regulator ERG
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
AID - 10.1074/jbc.274.24.17342 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 11;274(24):17342-52. doi: 10.1074/jbc.274.24.17342.