PMID- 10358091
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 24
DP  - 1999 Jun 11
TI  - A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export
      signal and CRM-1.
PG  - 17309-17
AB  - The HIV-1 Rev is a shuttling protein required for the nuclear export of unspliced
      and partially spliced viral mRNA. In this study, we have identified a new
      Rev-interacting protein, that specifically interacts with the Rev nuclear export 
      signal both in yeast and mammalian cells. This protein has features found in
      nucleoporins including many phenylalanine-glycine repeats, a very high serine
      content, a putative zinc finger, and a coiled-coil domain; we thus called it
      NLP-1 (nucleoporin-like protein 1). In addition, gene expression analysis and
      wheat germ agglutinin chromatography experiments suggested that NLP-1 is an
      ubiquitous O-glycosylated nuclear protein. Recently, a cellular factor called
      CRM-1 has been shown to be an essential nuclear export factor interacting
      directly with nuclear export signals including the Rev nuclear export signal in a
      RanGTP-dependent manner. We show here that NLP-1, like the previously described
      Rev-interacting protein hRIP/Rab and several nucleoporins, also interacts with
      CRM-1 both in yeast and mammalian cells.
FAU - Farjot, G
AU  - Farjot G
AD  - Laboratoire de Virologie, ENS-LYON, Unite INSERM U 412, 46, Allee d'Italie, 69364
      Lyon Cedex 07, France.
FAU - Sergeant, A
AU  - Sergeant A
FAU - Mikaelian, I
AU  - Mikaelian I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Gene Products, rev)
RN  - 0 (Karyopherins)
RN  - 0 (NUP42 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleocytoplasmic Transport Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Recombinant Proteins)
RN  - 0 (exportin 1 protein)
RN  - 0 (rev Gene Products, Human Immunodeficiency Virus)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
SB  - IM
MH  - Binding Sites
MH  - Biological Transport
MH  - Carrier Proteins/*metabolism
MH  - Cell Nucleus/*metabolism
MH  - Cloning, Molecular/methods
MH  - Gene Products, rev/genetics/*metabolism
MH  - HIV-1/*metabolism
MH  - Humans
MH  - *Karyopherins
MH  - Mutation
MH  - Nuclear Proteins/*metabolism
MH  - *Nucleocytoplasmic Transport Proteins
MH  - Protein Binding
MH  - *Receptors, Cytoplasmic and Nuclear
MH  - Recombinant Proteins/metabolism
MH  - Sequence Analysis, DNA
MH  - Tissue Distribution
MH  - Yeasts
MH  - ran GTP-Binding Protein
MH  - rev Gene Products, Human Immunodeficiency Virus
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
AID - 10.1074/jbc.274.24.17309 [doi]
AID - S0021-9258(19)72912-3 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 11;274(24):17309-17. doi: 10.1074/jbc.274.24.17309.