PMID- 10358071
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 24
DP  - 1999 Jun 11
TI  - Characterizing the interactions between the two subunits of the p101/p110gamma
      phosphoinositide 3-kinase and their role in the activation of this enzyme by G
      beta gamma subunits.
PG  - 17152-8
AB  - Recently, we have reported the purification and cloning of a novel G protein
      betagamma subunit-activated phosphoinositide 3-kinase from pig neutrophils. The
      enzyme comprises a p110gamma catalytic subunit and a p101 regulatory subunit. Now
      we have cloned the human ortholog of p101 and generated panels of p101 and
      p110gamma truncations and deletions and used these in in vitro and in vivo assays
      to determine the protein domains responsible for subunit interaction and
      activation by betagamma subunits. Our results suggest large areas of p101
      including both N- and C-terminal portions interact with the N-terminal half of
      p110gamma. While modifications of the N terminus of p110gamma could modulate its 
      intrinsic catalytic activity, binding to the N-terminal region of p101 was found 
      to be indispensable for activation of heterodimers with Gbetagamma.
FAU - Krugmann, S
AU  - Krugmann S
AD  - Signalling Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, United
      Kingdom. sonja.krugmann@bbsrc.ac.uk
FAU - Hawkins, P T
AU  - Hawkins PT
FAU - Pryer, N
AU  - Pryer N
FAU - Braselmann, S
AU  - Braselmann S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (G-protein Beta gamma)
RN  - 0 (GTP-Binding Protein beta Subunits)
RN  - 0 (GTP-Binding Protein gamma Subunits)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.1 (Heterotrimeric GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Dimerization
MH  - Enzyme Activation
MH  - *GTP-Binding Protein beta Subunits
MH  - *GTP-Binding Protein gamma Subunits
MH  - GTP-Binding Proteins/*metabolism
MH  - *Heterotrimeric GTP-Binding Proteins
MH  - Humans
MH  - Mutagenesis, Site-Directed
MH  - Neutrophils/enzymology
MH  - Phosphatidylinositol 3-Kinases/genetics/*metabolism
MH  - Point Mutation
MH  - Protein Binding
MH  - Recombinant Proteins/metabolism
MH  - Sequence Deletion
MH  - Swine
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
AID - 10.1074/jbc.274.24.17152 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 11;274(24):17152-8. doi: 10.1074/jbc.274.24.17152.