PMID- 10358058
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 24
DP  - 1999 Jun 11
TI  - Molecular characterization of cytosolic phospholipase A2-beta.
PG  - 17063-7
AB  - We have isolated a cDNA encoding a 1012-amino acid polypeptide cPLA2-beta, that
      has significant homology with cPLA2-alpha in both the calcium-dependent lipid
      binding domain as well as in the catalytic domain. Transient expression of
      cPLA2-beta cDNA in COS cells results in an increase in calcium-dependent
      phospholipase A1 (PLA1) and PLA2 activities compared with vector-transfected
      cells. cPLA2-beta is markedly less selective for cleavage at sn-2 as compared
      with cPLA2-alpha and cPLA2-gamma. Northern analysis reveals a cPLA2-beta
      transcript of 8 kilobase pairs that is expressed in all the human tissues
      examined. With the identification of cPLA2-beta, the newly defined cPLA2 family
      now comprises three members that may have dramatically different mechanisms for
      regulation of expression and enzymatic activation.
FAU - Song, C
AU  - Song C
AD  - Small Molecule Drug Discovery Group, Genetics Institute, Cambridge, Massachusetts
      02140, USA.
FAU - Chang, X J
AU  - Chang XJ
FAU - Bean, K M
AU  - Bean KM
FAU - Proia, M S
AU  - Proia MS
FAU - Knopf, J L
AU  - Knopf JL
FAU - Kriz, R W
AU  - Kriz RW
LA  - eng
SI  - GENBANK/AF121908
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.32 (Phospholipases A1)
RN  - EC 3.1.1.4 (Group IV Phospholipases A2)
RN  - EC 3.1.1.4 (Phospholipases A2)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Calcium/pharmacology
MH  - Cloning, Molecular
MH  - Cytosol/*enzymology
MH  - DNA, Complementary/genetics
MH  - Gene Library
MH  - Group IV Phospholipases A2
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phospholipases A/biosynthesis/drug effects/*genetics
MH  - Phospholipases A1
MH  - Phospholipases A2
MH  - Recombinant Proteins/biosynthesis
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - U937 Cells
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
AID - 10.1074/jbc.274.24.17063 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 11;274(24):17063-7. doi: 10.1074/jbc.274.24.17063.