PMID- 10358026
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 24
DP  - 1999 Jun 11
TI  - Adipose differentiation related protein (ADRP) expressed in transfected COS-7
      cells selectively stimulates long chain fatty acid uptake.
PG  - 16825-30
AB  - Adipose differentiation related protein (ADRP) is a 50-kDa novel protein cloned
      from a mouse 1246 adipocyte cDNA library, rapidly induced during adipocyte
      differentiation. We have examined ADRP function, and we show here that ADRP
      facilitates fatty acid uptake in COS cells transfected with ADRP cDNA. We
      demonstrate that uptake of long chain fatty acids was significantly stimulated in
      a time-dependent fashion in ADRP-expressing COS-7 cells compared with empty
      vector-transfected control cells. Oleic acid uptake velocity increased
      significantly in a dose-dependent manner in ADRP-expressing COS-7 cells compared 
      with control cells. The transport Km was 0.051 microM, and Vmax was 57.97
      pmol/10(5) cells/min in ADRP-expressing cells, and Km was 0.093 microM and Vmax
      was 20.13 pmol/10(5) cells/min in control cells. The oleate uptake measured at 4 
      degrees C was only 10% that at 37 degrees C. ADRP also stimulated uptake of
      palmitate and arachidonate but had no effect on uptake of medium chain fatty acid
      such as octanoic acid and glucose. These data suggest that ADRP specifically
      enhances uptake of long chain fatty acids by increasing the initial rate of
      uptake and provide novel information about ADRP function as a saturable transport
      component for long chain fatty acids.
FAU - Gao, J
AU  - Gao J
AD  - Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy,
      Baltimore, Maryland 21201-1180, USA.
FAU - Serrero, G
AU  - Serrero G
LA  - eng
GR  - R01DK 51463/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Caprylates)
RN  - 0 (Fatty Acids)
RN  - 0 (Membrane Proteins)
RN  - 0 (Perilipin-2)
RN  - 0 (Plin2 protein, mouse)
RN  - 0 (Recombinant Proteins)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 2UMI9U37CP (Oleic Acid)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - OBL58JN025 (octanoic acid)
SB  - IM
MH  - Adipose Tissue
MH  - Animals
MH  - Arachidonic Acid/metabolism
MH  - Biological Transport, Active
MH  - COS Cells
MH  - Caprylates/metabolism
MH  - Fatty Acids/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Membrane Proteins/*biosynthesis/genetics/isolation & purification
MH  - Mice
MH  - Oleic Acid/metabolism
MH  - Palmitic Acid/metabolism
MH  - Perilipin-2
MH  - Recombinant Proteins/biosynthesis
MH  - Transfection
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
AID - 10.1074/jbc.274.24.16825 [doi]
AID - S0021-9258(19)72847-6 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 11;274(24):16825-30. doi: 10.1074/jbc.274.24.16825.