PMID- 10358005
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 24
DP  - 1999 Jun 11
TI  - The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the
      translation termination signal to the 3'-Poly(A) tail of mRNA. Direct association
      of erf3/GSPT with polyadenylate-binding protein.
PG  - 16677-80
AB  - The mammalian GTP-binding protein GSPT, whose carboxyl-terminal sequence is
      homologous to the eukaryotic elongation factor EF1alpha, binds to the polypeptide
      chain releasing factor eRF1 to function as eRF3 in the translation termination.
      The amino-terminal domain of GSPT was, however, not required for the binding.
      Search for other GSPT-binding proteins in yeast two-hybrid screening system
      resulted in the identification of a cDNA encoding polyadenylate-binding protein
      (PABP), whose amino terminus is associating with the poly(A) tail of mRNAs
      presumably for their stabilization. The interaction appeared to be mediated
      through the carboxyl-terminal domain of PABP and the amino-terminal region of
      GSPT. Interestingly, multimerization of PABP with poly(A), which is ascribed to
      the action of its carboxyl-terminal domain, was completely inhibited by the
      interaction with the amino-terminal domain of GSPT. These results indicate that
      GSPT/eRF3 may play important roles not only in the termination of protein
      synthesis but also in the regulation of mRNA stability. Thus, the present study
      is the first report showing that GSPT/eRF3 carries the translation termination
      signal to 3'-poly(A) tail ubiquitously present in eukaryotic mRNAs.
FAU - Hoshino, S
AU  - Hoshino S
AD  - Department of Physiological Chemistry, Graduate School of Pharmaceutical
      Sciences, University of Tokyo, Tokyo 113-0033, Japan. hoshino@mol.f.u-tokyo.ac.jp
FAU - Imai, M
AU  - Imai M
FAU - Kobayashi, T
AU  - Kobayashi T
FAU - Uchida, N
AU  - Uchida N
FAU - Katada, T
AU  - Katada T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (ETF1 protein, human)
RN  - 0 (Peptide Termination Factors)
RN  - 0 (Poly(A)-Binding Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (peptide-chain-release factor 3)
SB  - IM
MH  - Eukaryotic Cells
MH  - Gene Library
MH  - Humans
MH  - Models, Genetic
MH  - Molecular Conformation
MH  - *Peptide Chain Termination, Translational
MH  - Peptide Termination Factors/*metabolism
MH  - Poly(A)-Binding Proteins
MH  - Protein Binding
MH  - Protein Biosynthesis
MH  - RNA, Messenger/*metabolism
MH  - RNA-Binding Proteins/*metabolism
MH  - Saccharomyces cerevisiae/genetics
EDAT- 1999/06/08 00:00
MHDA- 1999/06/08 00:01
CRDT- 1999/06/08 00:00
PHST- 1999/06/08 00:00 [pubmed]
PHST- 1999/06/08 00:01 [medline]
PHST- 1999/06/08 00:00 [entrez]
AID - 10.1074/jbc.274.24.16677 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Jun 11;274(24):16677-80. doi: 10.1074/jbc.274.24.16677.