PMID- 10356987
OWN - NLM
STAT- MEDLINE
DCOM- 19990630
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 451
IP  - 1
DP  - 1999 May 14
TI  - ZIP kinase identified as a novel myosin regulatory light chain kinase in HeLa
      cells.
PG  - 81-4
AB  - A novel myosin light chain kinase (MLCK) cDNA was isolated from a HeLa cell cDNA 
      library. The deduced amino acid sequence was identical to that of a
      zipper-interacting protein kinase (ZIPK) which mediates apoptosis [Kawai et al.
      (1998) Mol. Cell. Biol. 18, 1642-1651]. Here we found that HeLa ZIPK
      phosphorylated the regulatory light chain of myosin II (MRLC) at both serine 19
      and threonine 18 in a Ca2+/calmodulin independent manner. Phosphorylation of
      myosin II by HeLa ZIPK resulted in activation of actin-activated MgATPase
      activity of myosin II. HeLa ZIPK is the first non-muscle MLCK that phosphorylates
      MRLC at two sites.
FAU - Murata-Hori, M
AU  - Murata-Hori M
AD  - Department of Biological Science, Faculty of Science, Hiroshima University,
      Higashi-Hiroshima, Japan.
FAU - Suizu, F
AU  - Suizu F
FAU - Iwasaki, T
AU  - Iwasaki T
FAU - Kikuchi, A
AU  - Kikuchi A
FAU - Hosoya, H
AU  - Hosoya H
LA  - eng
SI  - GENBANK/AB022341
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (DNA, Complementary)
RN  - EC 2.7.11.1 (Death-Associated Protein Kinases)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.18 (Myosin-Light-Chain Kinase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Apoptosis Regulatory Proteins
MH  - Base Sequence
MH  - Calcium-Calmodulin-Dependent Protein Kinases
MH  - DNA, Complementary
MH  - Death-Associated Protein Kinases
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Myosin-Light-Chain Kinase/*metabolism
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/genetics/*metabolism
EDAT- 1999/06/05 00:00
MHDA- 1999/06/05 00:01
CRDT- 1999/06/05 00:00
PHST- 1999/06/05 00:00 [pubmed]
PHST- 1999/06/05 00:01 [medline]
PHST- 1999/06/05 00:00 [entrez]
AID - S0014-5793(99)00550-5 [pii]
AID - 10.1016/s0014-5793(99)00550-5 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 May 14;451(1):81-4. doi: 10.1016/s0014-5793(99)00550-5.