PMID- 10356364
OWN - NLM
STAT- MEDLINE
DCOM- 20000828
LR  - 20091119
IS  - 1522-4724 (Print)
IS  - 1522-4724 (Linking)
VI  - 1
IP  - 2
DP  - 1999 May
TI  - Expression of a Y559F mutant CSF-1 receptor in M1 myeloid cells: a role for Src 
      kinases in CSF-1 receptor-mediated differentiation.
PG  - 144-52
AB  - We have established two M1 myeloid cell lines, M1/WT cells overexpressing the 
      wild-type CSF-1 receptor and M1/Y559F cells expressing a specific tyrosine 
      mutant. M1/WT cells differentiated in response to CSF-1, with a reduction in 
      their proliferative capacity. CSF-1-mediated differentiation was partially 
      abrogated in the M1/Y559F cells, with a less marked reduction in proliferative 
      capacity. The Src tyrosine kinases c-Src, c-Yes, c-Fyn, and c-Hck were tyrosine 
      phosphorylated in the M1/WT cells in response to CSF-1 and bound to the WT CSF-1R 
      through their SH2 domains. Binding of the Src kinases to the CSF-1 receptor was 
      greatly reduced in the M1/Y559F cells. CSF-1-mediated activation of STAT3 was 
      also abrogated in the M1/Y559F cell line. Treatment of M1/WT cells with the Src 
      family inhibitor PP2 resulted in an inhibition of CSF-1-mediated differentiation, 
      equivalent to that observed in the M1/Y559F cells. These data suggest that the 
      reduced Src binding observed in the M1/Y559F cells may contribute to their 
      reduced ability to differentiate.
FAU - Marks, D C
AU  - Marks DC
AD  - Inflammation Research Centre, Department of Medicine, University of Melbourne, 
      Parkville, Victoria, Australia. x.csar@medicine.unimelb.edu.au
FAU - Csar, X F
AU  - Csar XF
FAU - Wilson, N J
AU  - Wilson NJ
FAU - Novak, U
AU  - Novak U
FAU - Ward, A C
AU  - Ward AC
FAU - Kanagasundarum, V
AU  - Kanagasundarum V
FAU - Hoffmann, B W
AU  - Hoffmann BW
FAU - Hamilton, J A
AU  - Hamilton JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol Res Commun
JT  - Molecular cell biology research communications : MCBRC
JID - 100889076
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Stat3 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.2 (src-Family Kinases)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - DNA-Binding Proteins/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Leukemia, Myeloid, Acute
MH  - Macrophage Colony-Stimulating Factor/pharmacology
MH  - Mice
MH  - Mutation
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - Receptor, Macrophage Colony-Stimulating Factor/genetics/*metabolism
MH  - STAT3 Transcription Factor
MH  - Signal Transduction
MH  - Trans-Activators/*metabolism
MH  - Tumor Cells, Cultured
MH  - src-Family Kinases/antagonists & inhibitors/*metabolism
EDAT- 1999/11/30 09:00
MHDA- 2000/09/02 11:01
CRDT- 1999/11/30 09:00
PHST- 1999/11/30 09:00 [pubmed]
PHST- 2000/09/02 11:01 [medline]
PHST- 1999/11/30 09:00 [entrez]
AID - S152247249990123X [pii]
AID - 10.1006/mcbr.1999.0123 [doi]
PST - ppublish
SO  - Mol Cell Biol Res Commun. 1999 May;1(2):144-52. doi: 10.1006/mcbr.1999.0123.