PMID- 10354480
OWN - NLM
STAT- MEDLINE
DCOM- 19990802
LR  - 20190909
IS  - 0925-4773 (Print)
IS  - 0925-4773 (Linking)
VI  - 82
IP  - 1-2
DP  - 1999 Apr
TI  - OTX2 homeodomain protein binds a DNA element necessary for interphotoreceptor
      retinoid binding protein gene expression.
PG  - 165-9
AB  - Transcription of the human interphotoreceptor retinoid binding protein (IRBP)
      gene is strictly tissue specific, being restricted to retinal photoreceptors and 
      pinealocytes. We have previously demonstrated that a sequence named A element, in
      the IRBP promoter is essential for IRBP gene transcription in vivo. Here we
      demonstrate that the human homeodomain protein OTX2 is present in nuclear
      extracts of IRBP expressing cells and specifically interacts with the IRBP A
      promoter element in vitro. OTX2, as well as CRX, a homeodomain protein very
      similar to OTX2, activates the human IRBP promoter in co-transfection
      experiments.
FAU - Bobola, N
AU  - Bobola N
AD  - Department of Oncology, Biology and Genetics, University of Genova, Genoa, Italy.
FAU - Briata, P
AU  - Briata P
FAU - Ilengo, C
AU  - Ilengo C
FAU - Rosatto, N
AU  - Rosatto N
FAU - Craft, C
AU  - Craft C
FAU - Corte, G
AU  - Corte G
FAU - Ravazzolo, R
AU  - Ravazzolo R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mech Dev
JT  - Mechanisms of development
JID - 9101218
RN  - 0 (DNA Primers)
RN  - 0 (Eye Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (OTX2 protein, human)
RN  - 0 (Otx Transcription Factors)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Retinol-Binding Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (interstitial retinol-binding protein)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cell Line
MH  - DNA/*genetics/*metabolism
MH  - DNA Primers/genetics
MH  - Eye Proteins/*genetics
MH  - HeLa Cells
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Otx Transcription Factors
MH  - Photoreceptor Cells/*metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Retina/metabolism
MH  - Retinol-Binding Proteins/*genetics
MH  - Spodoptera
MH  - Trans-Activators/genetics/*metabolism
MH  - Transfection
EDAT- 1999/06/04 00:00
MHDA- 1999/06/04 00:01
CRDT- 1999/06/04 00:00
PHST- 1999/06/04 00:00 [pubmed]
PHST- 1999/06/04 00:01 [medline]
PHST- 1999/06/04 00:00 [entrez]
AID - S0925477398001622 [pii]
AID - 10.1016/s0925-4773(98)00162-2 [doi]
PST - ppublish
SO  - Mech Dev. 1999 Apr;82(1-2):165-9. doi: 10.1016/s0925-4773(98)00162-2.