PMID- 10353696
OWN - NLM
STAT- MEDLINE
DCOM- 19990803
LR  - 20190921
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 11
IP  - 3
DP  - 1999 Mar
TI  - Activation of protein kinase A by dibutyryl cAMP treatment of NIH 3T3 cells
      inhibits proliferation but fails to induce Ser-133 phosphorylation and
      transcriptional activation of CREB.
PG  - 211-9
AB  - The cAMP analogue dibutyryl cAMP (dbcAMP) is often used to activate the protein
      kinase A pathway and to study the expression of cAMP-responsive genes. Here we
      show that in NIH 3T3 cells dbcAMP is able to activate PKA, but fails to stimulate
      expression of the cAMP-inducible c-fos gene. Co-expression of A-kinase anchoring 
      protein 75, previously shown to amplify cAMP signalling and to stimulate c-fos
      expression, could not restore cAMP responsiveness of the c-fos promoter.
      DbcAMP-induced activation of PKA may result in poor translocation of the
      catalytic sub-units of PKA to the nucleus, indicated by the lack of both Ser-133 
      phosphorylation of the cAMP-response element binding factor CREB and stimulation 
      of the transcriptional activity of this factor. DbcAMP treatment, however,
      inhibited cell proliferation. These results suggest that cAMP-mediated inhibition
      of proliferation may be independent of translocation of the catalytic sub-units
      into the nucleus.
FAU - Seternes, O M
AU  - Seternes OM
AD  - Department of Gene Biology, Institute of Medical Biology, University of Tromso,
      Norway.
FAU - Sorensen, R
AU  - Sorensen R
FAU - Johansen, B
AU  - Johansen B
FAU - Moens, U
AU  - Moens U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 1F7A44V6OU (Colforsin)
RN  - 452VLY9402 (Serine)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Bucladesine/*metabolism
MH  - Colforsin/pharmacology
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Mice
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Serine/*metabolism
MH  - Signal Transduction
MH  - Time Factors
MH  - *Transcriptional Activation
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - S0898-6568(98)00069-2 [pii]
AID - 10.1016/s0898-6568(98)00069-2 [doi]
PST - ppublish
SO  - Cell Signal. 1999 Mar;11(3):211-9. doi: 10.1016/s0898-6568(98)00069-2.