PMID- 10353695
OWN - NLM
STAT- MEDLINE
DCOM- 19990803
LR  - 20190921
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 11
IP  - 3
DP  - 1999 Mar
TI  - Prolactin-independent modulation of the beta-casein response element by Erk2 MAP 
      kinase.
PG  - 205-10
AB  - The MAP kinases have been suggested to play a role in intracellular signalling by
      PRL. A reporter gene construct, PRE3-CAT, which manifests PRL responsiveness
      through a Stat5-binding site (PRE), was induced by PRL in CHO cells expressing
      the PRL-R. A fusion protein (Gal4-Stat5(695)), containing the C-terminal domain
      of Stat5a (amino acids 695-794) linked to the DNA-binding domain of Gal4 (Gal4
      DBD), strongly activated transcription of a luciferase reporter gene. Therefore, 
      the Stat5 C-terminus, which contains a potential MAP kinase phosphorylation site,
      exhibits a modular transactivating function. A kinase-defective mutant of Erk2
      (iMAPK) caused a dose-dependent suppression of PRL-stimulated PRE3-CAT, and also 
      inhibited the induction of PRE3-CAT by Jak2 over-expression. Correspondingly,
      over-expression of the MAP kinase activator v-Src increased the PRL-stimulated
      level of PRE3-CAT. Gal4-Stat5(695) activity was not modulated by PRL or Jak2,
      consistent with the absence of the relevant tyrosine phosphorylation site at
      residue 694. Gal4-Stat5(695) was not inhibited by iMAPK, indicating that the
      C-terminal transactivation region of Stat5a is not sensitive to direct modulation
      of a MAP kinase pathway. These results suggest that alteration of Erk2 activity
      by growth factors may modulate PRL-induced gene expression by a mechanism
      upstream of Stat5.
FAU - Gao, J
AU  - Gao J
AD  - Department of Molecular and Cellular Physiology, University of Cincinnati, OH
      45267-0576, USA.
FAU - Horseman, N D
AU  - Horseman ND
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Caseins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Milk Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (STAT5 Transcription Factor)
RN  - 0 (Trans-Activators)
RN  - 9002-62-4 (Prolactin)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Janus Kinase 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*physiology
MH  - Caseins/*metabolism
MH  - Cricetinae
MH  - DNA-Binding Proteins/metabolism
MH  - Janus Kinase 2
MH  - *Milk Proteins
MH  - Mitogen-Activated Protein Kinase 1
MH  - Models, Biological
MH  - Prolactin/*physiology
MH  - Protein-Tyrosine Kinases/metabolism
MH  - *Proto-Oncogene Proteins
MH  - STAT5 Transcription Factor
MH  - Signal Transduction
MH  - Trans-Activators/metabolism
MH  - Transfection
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - S0898-6568(98)00067-9 [pii]
AID - 10.1016/s0898-6568(98)00067-9 [doi]
PST - ppublish
SO  - Cell Signal. 1999 Mar;11(3):205-10. doi: 10.1016/s0898-6568(98)00067-9.