PMID- 10353602
OWN - NLM
STAT- MEDLINE
DCOM- 19990617
LR  - 20090929
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 16
DP  - 1999 Apr 22
TI  - DEF-3(g16/NY-LU-12), an RNA binding protein from the 3p21.3 homozygous deletion
      region in SCLC.
PG  - 2589-97
AB  - DEF-3(g16/NY-LU-12) encodes a novel RNA binding protein isolated by positional
      cloning from an SCLC homozygous deletion region in 3p21.3 and, in parallel, as a 
      differentially expressed gene during myelopoiesis from FDCPmix-A4 cells.
      DEF-3(g16/NY-LU-12) is ubiquitously expressed during mouse embryogenesis and in
      adult organs while human hematopoietic tissues showed differential expression.
      The mouse and human proteins are highly conserved containing two RNA recognition 
      motifs (RRMs) and other domains associated with RNA binding and protein-protein
      interactions. A database search identified related proteins in human, rat, C.
      elegans and S. pombe including the 3p21.3 co-deleted gene, LUCA15. Recombinant
      proteins containing the RRMs of DEF-3(g16/NY-LU-12) and LUCA15 specifically bound
      poly(G) RNA homopolymers in vitro. These RRMs also show similarity to those of
      the Hu protein family. Since anti-Hu RRM domain antibodies are associated with an
      anti-tumor effect and paraneoplastic encephalomyelitis, we tested sera from Hu
      syndrome patients with the RRMs of DEF-3(g16/NY-LU-12) and LUCA15. These were
      non-reactive. Thus, DEF-3(g16/NY-LU-12) and LUCA15 represent members of a novel
      family of RNA binding proteins with similar expression patterns and in vitro RNA 
      binding characteristics. They are co-deleted in some lung cancers and
      immunologically distinct from the Hu proteins.
FAU - Drabkin, H A
AU  - Drabkin HA
AD  - Division of Medical Oncology, University of Colorado Health Sciences Center,
      Denver 80262, USA.
FAU - West, J D
AU  - West JD
FAU - Hotfilder, M
AU  - Hotfilder M
FAU - Heng, Y M
AU  - Heng YM
FAU - Erickson, P
AU  - Erickson P
FAU - Calvo, R
AU  - Calvo R
FAU - Dalmau, J
AU  - Dalmau J
FAU - Gemmill, R M
AU  - Gemmill RM
FAU - Sablitzky, F
AU  - Sablitzky F
LA  - eng
SI  - GENBANK/AJ006486
SI  - GENBANK/X96701
GR  - CA58187/CA/NCI NIH HHS/United States
GR  - P30 CA 46934/CA/NCI NIH HHS/United States
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (RBM6 protein, human)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Carcinoma, Small Cell/*genetics
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 3
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Evolution, Molecular
MH  - Humans
MH  - Lung Neoplasms/*genetics
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - RNA-Binding Proteins/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion
MH  - Sequence Homology, Amino Acid
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - 10.1038/sj.onc.1202601 [doi]
PST - ppublish
SO  - Oncogene. 1999 Apr 22;18(16):2589-97. doi: 10.1038/sj.onc.1202601.