PMID- 10352938
OWN - NLM
STAT- MEDLINE
DCOM- 19990812
LR  - 20061115
IS  - 1018-4813 (Print)
IS  - 1018-4813 (Linking)
VI  - 7
IP  - 4
DP  - 1999 May-Jun
TI  - A comparison of genomic structures and expression patterns of two closely related
      flanking genes in a critical lung cancer region at 3p21.3.
PG  - 478-86
AB  - In the search for a tumour suppressor gene in the 3p21.3 region we isolated two
      genes, RBM5 and RBM6. Gene RBM5 maps to the region which is homozygously deleted 
      in the small cell lung cancer cell line GLC20; RBM6 crosses the telomeric
      breakpoint of this deletion. Sequence comparison revealed that at the amino acid 
      level both genes show 30% identity. They contain two zinc finger motifs, a
      bipartite nuclear signal and two RNA binding motifs, suggesting that the proteins
      for which RBM5 and RBM6 are coding have a DNA/RNA binding function and are
      located in the nucleus. Northern and Southern analysis did not reveal any
      abnormalities. By SSCP analysis of 16 lung cancer cell lines we found only in
      RBM5 a single presumably neutral mutation. By RT-PCR we demonstrated the
      existence of two alternative splice variants of RBM6, one including and one
      excluding exon 5, in both normal lung tissue and lung cancer cell lines.
      Exclusion of exon 5 results in a frameshift which would cause a truncated protein
      of 520 amino acids instead of 1123 amino acids. In normal lung tissue, the
      relative amount of the shorter transcript was much greater than that in the lung 
      tumour cell lines, which raises the question whether some tumour suppressor
      function may be attributed to the derived shorter protein.
FAU - Timmer, T
AU  - Timmer T
AD  - Department of Medical Genetics, University of Groningen, The Netherlands.
FAU - Terpstra, P
AU  - Terpstra P
FAU - van den Berg, A
AU  - van den Berg A
FAU - Veldhuis, P M
AU  - Veldhuis PM
FAU - Ter Elst, A
AU  - Ter Elst A
FAU - Voutsinas, G
AU  - Voutsinas G
FAU - Hulsbeek, M M
AU  - Hulsbeek MM
FAU - Draaijers, T G
AU  - Draaijers TG
FAU - Looman, M W
AU  - Looman MW
FAU - Kok, K
AU  - Kok K
FAU - Naylor, S L
AU  - Naylor SL
FAU - Buys, C H
AU  - Buys CH
LA  - eng
SI  - GENBANK/AF091263
SI  - GENBANK/AF091264
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Hum Genet
JT  - European journal of human genetics : EJHG
JID - 9302235
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proteins)
RN  - 0 (RBM5 protein, human)
RN  - 0 (RBM6 protein, human)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carcinoma, Small Cell/genetics
MH  - Cell Cycle Proteins
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - DNA, Neoplasm/analysis
MH  - DNA-Binding Proteins/chemistry/*genetics/metabolism
MH  - Exons/genetics
MH  - *Genes, Tumor Suppressor
MH  - Humans
MH  - Introns/genetics
MH  - Lung Neoplasms/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Polymorphism, Single-Stranded Conformational
MH  - Proteins/chemistry/*genetics/metabolism
MH  - RNA-Binding Proteins/chemistry/*genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Proteins
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - 10.1038/sj.ejhg.5200334 [doi]
PST - ppublish
SO  - Eur J Hum Genet. 1999 May-Jun;7(4):478-86. doi: 10.1038/sj.ejhg.5200334.