PMID- 10352232
OWN - NLM
STAT- MEDLINE
DCOM- 19990719
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 232
IP  - 2
DP  - 1999 May 31
TI  - Cloning, central nervous system expression and chromosomal mapping of the mouse
      PAK-1 and PAK-3 genes.
PG  - 209-15
AB  - Two cDNAs encoding PAK kinases were isolated from a mouse embryo library by
      screening with a PCR-generated probe derived from the kinase domain of a rat PAK 
      kinase. These cDNAs, designated PAK-1 and PAK-3, encode mouse PAK kinases of 545 
      and 544 amino acids, respectively. Both proteins possess an N-terminal Cdc42/Rac 
      interacting binding domain (CRIB) and a C-terminal serine/threonine kinase
      domain. Comparison of the two mouse PAK kinases revealed that the proteins show
      87% amino acid identity. Northern analysis of a multiple mouse tissue blot with a
      PAK-1 probe detected a 3.0kb transcript that was almost exclusively expressed in 
      the brain and spinal cord compared to other tissues such as lung, liver and
      kidney. A similar pattern of central nervous system tissue expression of PAK-3
      transcripts of 3.6 and 8kb was also observed. Analysis of two multilocus genetic 
      crosses localized Pak1 and Pak3 to a position on chromosome 7 and X,
      respectively. The high level of PAK-1 and PAK-3 kinase expression in the mouse
      brain and spinal cord suggests a potentially important role for these kinases in 
      the control of the cellular architecture and/or signaling in the central nervous 
      system.
FAU - Burbelo, P D
AU  - Burbelo PD
AD  - Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC
      20007, USA. burbelop@medlib.georgetown.edu
FAU - Kozak, C A
AU  - Kozak CA
FAU - Finegold, A A
AU  - Finegold AA
FAU - Hall, A
AU  - Hall A
FAU - Pirone, D M
AU  - Pirone DM
LA  - eng
SI  - GENBANK/AF082077
SI  - GENBANK/AF082297
GR  - R29-CA77459-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - EC 2.7.11.1 (Pak1 protein, mouse)
RN  - EC 2.7.11.1 (Pak1 protein, rat)
RN  - EC 2.7.11.1 (Pak3 protein, mouse)
RN  - EC 2.7.11.1 (Pak3 protein, rat)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (p21-Activated Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain Chemistry/*genetics
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - *Gene Expression
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity/genetics
MH  - Protein-Serine-Threonine Kinases/biosynthesis/*genetics
MH  - Sequence Homology, Amino Acid
MH  - p21-Activated Kinases
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - S0378111999001109 [pii]
AID - 10.1016/s0378-1119(99)00110-9 [doi]
PST - ppublish
SO  - Gene. 1999 May 31;232(2):209-15. doi: 10.1016/s0378-1119(99)00110-9.