PMID- 10352183
OWN - NLM
STAT- MEDLINE
DCOM- 19990726
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 27
IP  - 12
DP  - 1999 Jun 15
TI  - Interaction between human topoisomerase I and a novel RING finger/arginine-serine
      protein.
PG  - 2538-44
AB  - The N-terminus of human topoisomerase I participates in the binding of this
      enzyme to helicases and other proteins. Using the N-terminal 250 amino acids of
      human topoisomerase I and a yeast two-hybrid/ in vitro binding screen, a novel
      arginine-serine-rich peptide was identified as a human topoisomerase I-binding
      protein. The corresponding full-length protein, named topors, contains a
      consensus RING zinc finger domain and nuclear localization signals in addition to
      the arginine-serine-rich region. The RING finger domain of topors is homologous
      to a similar domain in a family of viral proteins that are involved in the
      regulation of viral transcription. When expressed in HeLa cells as a green
      fluorescent protein fusion, topors localizes in the nucleus in a punctate pattern
      and co-immunoprecipitates with topoisomerase I. These data suggest that topors is
      involved in trans-cription, possibly recruiting topoisomerase I to RNA polymerase
      II transcriptional complexes.
FAU - Haluska, P Jr
AU  - Haluska P Jr
AD  - Departments of Pharmacology and Medicine, Robert Wood Johnson Medical School, The
      Cancer Institute of New Jersey, University of Medicine and Dentistry of New
      Jersey, New Brunswick, NJ 08901, USA.
FAU - Saleem, A
AU  - Saleem A
FAU - Rasheed, Z
AU  - Rasheed Z
FAU - Ahmed, F
AU  - Ahmed F
FAU - Su, E W
AU  - Su EW
FAU - Liu, L F
AU  - Liu LF
FAU - Rubin, E H
AU  - Rubin EH
LA  - eng
SI  - GENBANK/AF098300
GR  - CA70981/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.2.27 (TOPORS protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 5.99.1.2 (DNA Topoisomerases, Type I)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Carrier Proteins
MH  - Cell Nucleus/metabolism
MH  - DNA Topoisomerases, Type I/*metabolism
MH  - DNA, Complementary/analysis
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Neoplasm Proteins
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Precipitin Tests
MH  - Saccharomyces cerevisiae
MH  - Sequence Homology, Amino Acid
MH  - *Transcription Factors
MH  - U937 Cells
MH  - Ubiquitin-Protein Ligases
MH  - Zinc Fingers
PMC - PMC148458
EDAT- 1999/06/03 10:00
MHDA- 2001/03/28 10:01
CRDT- 1999/06/03 10:00
PHST- 1999/06/03 10:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/06/03 10:00 [entrez]
AID - gkc403.htm [pii]
AID - 10.1093/nar/27.12.2538 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1999 Jun 15;27(12):2538-44. doi: 10.1093/nar/27.12.2538.