PMID- 10350657
OWN - NLM
STAT- MEDLINE
DCOM- 19990702
LR  - 20201209
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1427
IP  - 3
DP  - 1999 May 24
TI  - Partial nuclear localization of a bovine phosphoprotein, BCNT, that includes a
      region derived from a LINE repetitive sequence in Ruminantia.
PG  - 408-16
AB  - BCNT, named after Bucentaur, is a protein that contains a 324-amino-acid region
      derived from part of a long interspersed DNA sequence element (LINE) in
      Ruminantia. However, the unique portion is completely missing in human and mouse 
      BCNTs. Since no significant information on their function has been obtained by
      homology search, we at first examined cellular localization and biochemical
      characteristics of bovine BCNT to get a hint on its function. Subcellular
      fractionation and immunohistochemical analyses using a normal bovine epithelial
      cell line and bovine brain revealed that a significant amount of bovine BCNT is
      localized in the nuclei, while the major portion is present in the cytosol.
      Furthermore, it was shown that bovine BCNT is a phosphoprotein and that both
      bovine and human BCNTs are phosphorylated by casein kinase II in vitro. These
      results show that BCNTs consist of a unique family, probably a substrate of
      casein kinase II, which may contribute further to the understanding of gene
      evolution.
FAU - Iwashita, S
AU  - Iwashita S
AD  - Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo
      194, Japan. siwast@libra.ls.m-kagaku.co.jp
FAU - Nobukuni, T
AU  - Nobukuni T
FAU - Tanaka, S
AU  - Tanaka S
FAU - Kobayashi, M
AU  - Kobayashi M
FAU - Iwanaga, T
AU  - Iwanaga T
FAU - Tamate, H B
AU  - Tamate HB
FAU - Masui, T
AU  - Masui T
FAU - Takahashi, I
AU  - Takahashi I
FAU - Hashimoto, K
AU  - Hashimoto K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (CFDP1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/*metabolism
MH  - Cattle
MH  - Cell Fractionation
MH  - Cell Line
MH  - Cell Nucleus/*metabolism
MH  - DNA/chemistry
MH  - Deer
MH  - Immunohistochemistry
MH  - Kidney/*metabolism
MH  - Liver/metabolism
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - Phosphoproteins/*analysis/genetics/isolation & purification
MH  - Precipitin Tests
MH  - Repetitive Sequences, Nucleic Acid
MH  - Ruminants/*genetics
MH  - Sequence Homology, Amino Acid
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - S0304-4165(99)00049-5 [pii]
AID - 10.1016/s0304-4165(99)00049-5 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 May 24;1427(3):408-16. doi:
      10.1016/s0304-4165(99)00049-5.