PMID- 10350639
OWN - NLM
STAT- MEDLINE
DCOM- 19990712
LR  - 20200204
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 69
IP  - 1
DP  - 1999 May 21
TI  - Molecular cloning and characterization of two putative G protein-coupled
      receptors which are highly expressed in the central nervous system.
PG  - 73-83
AB  - We have cloned from a rat hypothalamic cDNA library two closely related G
      protein-coupled receptors (GPCRs) which we have designated GPCR/CNS1 and
      GPCR/CNS2. The peptide sequences of these two G protein-coupled receptors shared 
      42% identity with each other and were next most closely related to the endothelin
      receptors and the bombesin-like peptide receptors (approximately 25% identity).
      Northern blot analysis showed that both GPCR/CNS1 and GPCR/CNS2 were very highly 
      expressed in rat brain. In situ hybridization of rat brain demonstrated broad
      distribution of both receptors throughout the central nervous system. GPCR/CNS1
      appeared to be expressed primarily in glial cells of the fiber tracts, while
      GPCR/CNS2 was expressed primarily in cells of the gray matter. The different
      distribution patterns of these two receptors in rat brain suggests distinct
      functional roles for each receptor in the central nervous system. Expression of
      these two receptors in Xenopus oocytes showed no response to any known endothelin
      and bombesin-like peptides. Therefore, the endogenous ligands and physiological
      significance of GPCR/CNS1 and GPCR/CNS2 remain to be elucidated, but may be
      related to the endothelins or bombesins. The very abundant expression in brain by
      these two receptors, however, suggests that they play important roles in the
      central nervous system.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Leng, N
AU  - Leng N
AD  - Division of Neuroscience Oregon Regional Primate Research Center, Beaverton, OR
      97006, USA.
FAU - Gu, G
AU  - Gu G
FAU - Simerly, R B
AU  - Simerly RB
FAU - Spindel, E R
AU  - Spindel ER
LA  - eng
SI  - GENBANK/AF087946
SI  - GENBANK/AF087947
GR  - NS35580/NS/NINDS NIH HHS/United States
GR  - RR00163/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (DNA, Complementary)
RN  - 0 (Gpr37 protein, rat)
RN  - 0 (Gpr37l1 protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 28S)
RN  - 0 (Receptor, Endothelin B)
RN  - 0 (Receptors, Bombesin)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Endothelin)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 5.2.1.8 (Peptidylprolyl Isomerase)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Brain Chemistry/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - GTP-Binding Proteins/*genetics
MH  - In Situ Hybridization
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*genetics
MH  - Oocytes/physiology
MH  - Peptidylprolyl Isomerase/genetics
MH  - RNA, Messenger/analysis
MH  - RNA, Ribosomal, 18S/analysis
MH  - RNA, Ribosomal, 28S/analysis
MH  - Rats
MH  - Receptor, Endothelin B
MH  - Receptors, Bombesin/genetics
MH  - Receptors, Cell Surface/*genetics
MH  - Receptors, Endothelin/genetics
MH  - *Receptors, G-Protein-Coupled
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Xenopus laevis
EDAT- 1999/06/03 00:00
MHDA- 1999/06/03 00:01
CRDT- 1999/06/03 00:00
PHST- 1999/06/03 00:00 [pubmed]
PHST- 1999/06/03 00:01 [medline]
PHST- 1999/06/03 00:00 [entrez]
AID - S0169328X99000923 [pii]
AID - 10.1016/s0169-328x(99)00092-3 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1999 May 21;69(1):73-83. doi:
      10.1016/s0169-328x(99)00092-3.